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United States Patent | 6,218,529 |
An ,   et al. | April 17, 2001 |
Disclosed are diagnostic techniques for the detection of human prostate, bladder and breast cancer. Genetic probes and methods useful in monitoring the progression and diagnosis of prostate, bladder and breast cancer are described. The invention relates particularly to probes and methods for evaluating the presence of RNA species that are differentially expressed in prostate, bladder and breast cancer compared to normal human prostate, benign prostatic hyperplasia, or normal bladder or breast tissue.
Inventors: | An; Gang (Oklahoma City, OK); O'Hara; S. Mark (Oklahoma City, OK); Ralph; David (Edmund, OK); Veltri; Robert (Oklahoma City, OK) |
Assignee: | UroCor, Inc. (Oklahoma City, OK) |
Appl. No.: | 097199 |
Filed: | June 12, 1998 |
Current U.S. Class: | 536/24.33; 435/6; 536/23.1; 536/23.4; 536/23.5; 536/24.31 |
Intern'l Class: | C07H 021/04; C12Q 001/68 |
Field of Search: | 536/24.33,24.31,23.1,23.4,23.5 435/6 |
5262311 | Nov., 1993 | Pardee et al. | 435/91. |
5342762 | Aug., 1994 | Mosher et al. | 435/69. |
5455158 | Oct., 1995 | Vogel et al. | 435/7. |
5487985 | Jan., 1996 | McClelland et al. | 435/91. |
5550214 | Aug., 1996 | Eberlein et al. | 530/328. |
5599672 | Feb., 1997 | Liang et al. | 435/6. |
5633161 | May., 1997 | Shyjan | 435/325. |
5639656 | Jun., 1997 | Wright, Jr. | 435/344. |
5665547 | Sep., 1997 | Pardee et al. | 435/6. |
Foreign Patent Documents | |||
WO 94/10343 | May., 1994 | WO. |
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UC 25 SEQ ID NO:1 UC 27 SEQ ID NO:2 UC 28 SEQ ID NO:3, SEQ ID NO:83, SEQ ID NO:85 UC 31 SEQ ID NO:4 UC 32 SEQ ID NO:7 (fibronectin) UC 33 SEQ ID NO:5 Cyclin A SEQ ID NO:8 truncated neu SEQ ID NO:9 UC 38 SEQ ID NO:10 UC 40 SEQ ID NO:11 UC 41 SEQ ID NO:12 UC 43 SEQ ID NO:19 UC 47 SEQ ID NO:47 (prostatic acid phosphatase) UC 201 SEQ ID NO:13 UC 204 SEQ ID NO:20 UC 205 SEQ ID NO:14 (Hek) UC 207 SEQ ID NO:15 UC 209 SEQ ID NO:16 UC 210 SEQ ID NO:17 UC 211 SEQ ID NO:21 UC 212 SEQ ID NO:22 UC 213 SEQ ID NO:23 UC 214 SEQ ID NO:45 UC 215 SEQ ID NO:46
1. UC 25 2. UC 27 3. UC 28 4. UC 31 5. UC 33 6. UC 214 primer 7. UC 32 fibronectin 8. Cyclin A 9. Truncated NEU 10. UC 38 11. UC 40 12. UC 41 13. UC 201 14. UC 205 (human HEK) 15. UC 207 16. UC 209 17. UC 210 18. UC 214 primer 19. UC 43 20. UC 204 21. UC 211 22. UC 212 23. UC 213 24. UC 215 primer 25. UC 215 primer 26. cyclin A primer 27. cyclin A primer 28. UC 25 primer 29. UC 25 primer 30. UC 27 primer 31. UC 27 primer 32. UC 28 primer 33. UC 28 primer 34. UC 31 primer 35. UC 31 primer 36. UC 32 primer 37. UC 32 primer 38. UC 33 primer 39. UC 33 primer 40. .beta.-Actin primer 41. .beta.-Actin primer 42. 5' primer UC 28 mRNA 43. 3' primer UC 28 2.1 kb mRNA 44. NET T3' primer 45. UC 214 46. UC 215 47. UC 47 (prostatic acid phosphatase) 48. Amino Acid sequence of UC 47 49. UC 38 primer 50. UC 38 primer 51. UC 40 primer 52. UC 40 primer 53. UC 41 primer 54. UC 41 primer 55. UC 28 ISH probe 56. UC 28 antigenic peptide 57. UC 43 primer 58. UC 43 primer 59. UC 47 primer 60. UC 47 primer 61. UC 201 primer 62. UC 201 primer 63. UC 204 primer 64. UC 204 primer 65. UC 205 primer 66. UC 205 primer 67. UC 207 primer 68. UC 207 primer 69. UC 209 primer 70. UC 209 primer 71. UC 210 primer 72. UC 210 primer 73. UC 211 primer 74. UC 211 primer 75. UC 212 primer 76. UC 212 primer 77. UC 213 primer 78. UC 213 primer 79. PSA primer 80. PSA primer 81. .beta.-Actin primer 82. .beta.-Actin primer 83. UC 28 84. UC 28 amino sequence 85. UC 28 86. UC 28 amino acid sequence 87. primer UC 28 2.5 kb mRNA
TABLE 1 Amino Acids Codons Alanine Ala A GCA GCC GCG GCU Cysteine Cys C UGC UGU Aspartic acid Asp D GAC GAU Glutamic acid Glu E GAA GAG Phenylalanine Phe F UUC UUU Glycine Gly G GGA GGC GGG GGU Histidine His H CAC CAU Isoleucine Ile I AUA AUC AUU Lysine Lys K AAA AAG Leucine Leu L UUA UUG CUA CUC CUG CUU Methionine Met M AUG Asparagine Asn N AAC AAU Proline Pro P CCA CCC CCG CCU Glutamine Gln Q CAA CAG Arginine Arg R AGA AGG CGA CGC CGG CGU Serine Ser S AGC AGU UCA UCC UCG UCU Threonine Thr T ACA ACC ACG ACU Valine Val V GUA GUC GUG GUU Tryptophan Trp W UGG Tyrosine Tyr Y UAC UAU
TABLE 2 Raw Numerical Data Captured on the IS1000 and Normalization by Comparison to B-Actin Raw Data Raw Data Normalized Type of Raw Data corrected for Normalizing for Hek Data for Hek Tissue B-Actin background Statistic (UC205) (UC205) Normal Pool 1 16 11 1.42 Normal Pool 2 35 30 0.52 Total normal 25.5 20.5 0.76 22 16.72 Pool BPH1 13 8 1.96 37 72.52 BPH2 27 22 0.71 10 17.1 BPH3 36 31 0.5 44 22 BPH4 1 31 31 BPH5 18 13 1.2 24 28.8 BPH6 15 10 1.56 41 63.96 BPH7 17 12 1.3 51 66.3 BPH8 21 16 0.975 39 38 BPH9 11 6 2.6 50 130 BPH10 17 12 1.3 14 18.2 BPH Pool 19.4 14.4 1.08 Cancer1 13 8 1.96 Cancer2 18 13 1.2 Cancer3 22 17 0.92 Cancer4 25 20 0.78 Cancer5 29 24 0.65 Cancer6 1 Cancer7 22 17 0.92 Cancer8 22 17 0.92 Cancer9 15 10 1.56 Cancer10 16 11 1.42 Cancer11 11 6 2.6 Cancer(Met)12 34 29 0.54 Cancer Pool 20.6 15.7 1 41 41 No template 5 0 Background 5 0 Total 497.9 377.9 Average 20.6 15.6
TABLE 3 Genes Whose mRNAs have Abundances that Vary in Prostate Cancer Relative to Normal and Benign Glands Name of cDNA Sequence Confirmed Previously SEQ Fragment Determined by RT-PCR Known ID NO: UC Band #25 Yes Yes No 1 UC Band #27 Yes Yes No 2 UC Band #28 Yes Yes No 3, 83, 84 UC Band #31 Yes Yes No 4 UC Band #32 Yes Yes fibronectin 7 US Band #33 Yes Yes No 5 Cyclin A Yes Yes Cyclin A 8 Trunc. Yes Yes Tru. HER2/neu 9 HER2/neu UC Band #38 Yes Yes No 10 UC Band #40 Yes Yes No 11 UC Band #41 Yes Yes No 12 UC Band #43 Yes Yes No 19 UC Band #47 Yes Yes Prostatic Acid 47 Phosphatase UC Band #201 Yes Yes No 13 UC Band #204 Yes Yes GB #Z28521 20 and GB #D42055 UC Band #205 Yes Yes Humhek 14 UC Band #207 Yes Yes No 15 UC Band #209 Yes Yes No 16 UC Band #210 Yes Yes No 17 UC Band #211 Yes Yes No 21 UC Band #212 Yes Yes No 22 UC Band #213 Yes Yes GB #T07736 23 UC Band #214 Yes Yes No 45 UC Band #215 Yes Yes No 46