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United States Patent | 6,190,919 |
Mastny ,   et al. | February 20, 2001 |
A system for controlling the deglycerolization of red blood cells includes a cell sorter having multiple fluid channels each having a unique cross-sectional area for directing a fluid mixture consisting essentially of a saline solution and a plasma solution having glycerized red blood cell products through one or more of the fluid channels based on the sizes of the red blood cell products. An optical energy source illuminates the fluid mixture in the cell sorter, whereupon an optical detector generates a data signal in response to receiving light signals that propagate through the fluid mixture. A processor generates a control signal in response to receiving the data signal that is used by a servo-controlled device to control the ratio of the saline and plasma solutions in the fluid mixture so that the red blood cell products substantially flow only through one or more of the fluid channels having particular cross-sectional areas.
Inventors: | Mastny; Gary F. (San Diego, CA); Copeland; Hugh D. (Chula Vista, CA); Patterson; Andrew E. (San Diego, CA) |
Assignee: | The United States of America as represented by the Secretary of the Navy (Washington, DC) |
Appl. No.: | 295875 |
Filed: | April 21, 1999 |
Current U.S. Class: | 436/63; 356/39; 356/317; 356/318; 356/335; 356/336; 356/337; 422/58; 422/73; 422/81; 422/82.05; 436/52; 436/164; 436/165 |
Intern'l Class: | G01N 033/48 |
Field of Search: | 436/63,164,165,174,175,52 422/58,73,81,82.05,82.09,102 356/39,317,318,335,336,337,244,246 |
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TABLE 1 Channel Width (.mu.) Height (.mu.) 31 H .apprxeq. 3 mm G .apprxeq. 1.4-1.6 32 F .apprxeq. 18-22 G .apprxeq. 1.4-1.6 34 E .apprxeq. 12-15 G .apprxeq. 1.4-1.6 36 D .apprxeq. 8-9 G .apprxeq. 1.4-1.6 38 C .apprxeq. 5-7 G .apprxeq. 1.4-1.6 40 B .apprxeq. 2-3 G .apprxeq. 1.4-1.6 42 A < 2 G .apprxeq. 1.4-1.6