Back to EveryPatent.com
United States Patent | 6,184,449 |
Ranu | February 6, 2001 |
A gene which encodes an ACC synthase is isolated from rose plants, specifically Rosa (cardinal red). This gene is modified for expression in transgenic plants. Isolation of high quality mRNA for gene isolation is achieved through use and adaptation of a 2-butoxyethanol precipitation technique using large amount of initial tissue in order to achieve critical mass for precipitation.
Inventors: | Ranu; Rajinder S (Fort Collins, CO) |
Assignee: | Tagawa Greenhouses, Inc. (Brighton, CO) |
Appl. No.: | 171482 |
Filed: | October 19, 1998 |
PCT Filed: | September 30, 1997 |
PCT NO: | PCT/US97/17644 |
371 Date: | October 19, 1998 |
102(e) Date: | October 19, 1998 |
PCT PUB.NO.: | WO98/14465 |
PCT PUB. Date: | April 9, 1998 |
Current U.S. Class: | 800/323; 435/320.1; 435/419; 536/23.2; 536/23.6; 800/283; 800/286 |
Intern'l Class: | A01H 005/00; C12N 005/14; C12N 015/29; C12N 015/52; C12N 015/82 |
Field of Search: | 435/69.1,419,320.1,468 536/23.2,23.6 800/278,283,286,287,298,323 |
4237224 | Dec., 1980 | Cohen et al. | 435/68. |
4468464 | Aug., 1984 | Cohen et al. | 435/317. |
4740463 | Apr., 1988 | Weinberg et al. | 435/172. |
4740470 | Apr., 1988 | Cohen et al. | 435/172. |
4782022 | Nov., 1988 | Puhler et al. | 435/172. |
4801540 | Jan., 1989 | Hiatt et al. | 435/172. |
4962028 | Oct., 1990 | Bedbrook et al. | 435/172. |
5107065 | Apr., 1992 | Shewmaker et al. | 800/205. |
5123951 | Jun., 1992 | See et al. | 71/86. |
5208149 | May., 1993 | Inouye | 435/91. |
5272065 | Dec., 1993 | Inouye et al. | 435/91. |
5378619 | Jan., 1995 | Rogers | 435/172. |
5416250 | May., 1995 | Ferro et al. | 800/205. |
5565347 | Oct., 1996 | Fillatti et al. | 435/172. |
5674731 | Oct., 1997 | Lin et al. | 435/240. |
5689055 | Nov., 1997 | Meyerowitz et al. | 800/205. |
5723766 | Mar., 1998 | Theologis et al. | 800/205. |
5759829 | Jul., 1998 | Shewmaker et al. | 435/172. |
5824868 | Oct., 1998 | Meyerowitz et al. | 800/205. |
5824875 | Oct., 1998 | Ranu | 800/205. |
Oeller PW, et al. "Reversible inhibition of tomato fruit senescence by antisense RNA." Science 254:437-439, Oct. 1991. Firoozabady E, et al. "Regeneration of transgenic rose (Rosa hybrida) plants from embryogenic tissue." Bio/Technol. 12: 609-613, Oct. 1991. "The Role of Ethylene in Regulating Growth of Deepwater Rice," H. Kende, S. Hoffmann-Benning and M. Sauter MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, USA, J.C.Pech et al. (eds.), Cellular and Molecular Aspects of the Plant Hormone Ethylene, 329-334, 1993, Kluwer Academic Publishers. "Temporal and Spatial Regulation of 1-Aminocyclopropane-1Carboxylate Oxidase in the Pollination-Induced Senescence of Orchid Flowers", Jeanette A. Nadeau, Xian Sheng Zhang, Helen Nair, and Sharman D. O'Neill*, Division of Biological Science, Section of Plant Biology, University of California at Davis, Davis, CA, Plant Physiol. (1993) 103:31-9. "A Flower Senescence-Related mRNA from Carnation Shares Sequence Similarity with Fruit Ripening-Related mRNAs Involved in Ethylene Biosynthesis," Hong Wang and William R. Woodson, Department of Horticulture, Purdue University, West Lafayette, IN, Plant Physiol (1991) 1000-1001. "Ethylene and Flower Senescence," Michael S. Reid & Men-Jen Wu, Department of Environmental Horticulture, University of California, Davis, CA, Plant Growth Regulation II: 37-43, 1992. "Cloning and Characterization of the cDNA encoding 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase from Pelargonium Hortorum-sincerity", Jianguo Fan, Dong Wang and Rajinder Ranu, Department of Plant Pathology and Weed Science, Colorado State University, Fort Collins, RNA 2368, The FASEB Journal, Apr. 30, 1996, vol. 10, No. 6. "Expression of Two ACC Synthase mRNAs In Carnation Flower Parts During Aging and Following Treatment With Ethylene," Hans Henskens, Dianne Somhorst and Ernest J. Woltering, Agrotechnological Research Institute (ATO-DLO) P.O. Box 17, 6700 AA Wageningen, The Netherlands, J.C. Pech et al. (eds), Cellular and Molecular Aspects of the Plant Hormone Ethylene, 323-324, 1993 Kluwer Academic Publishers. "Nucleotide Sequence Of A cDNA Encoding 1-Aminocyclopropane-1-Carboxylate Oxidase from Senescing Orchid Petals," Jeanette A. Nadeau and Sharman D. O'Neill, Division of Biological Science, Section of Plant Biology, University of California, Davis, CA., Plant Physiol, (1995) 108: 833-834. "Nucleotide Sequence of a cDNA Encoding the Ethylene-Forming Enzyme from Petunia Corollas," Hong Wang and William R. Woodson, Department of Horticulture, Purdue University, West Lafayette, IN, Plant Physiol, (1992), 100, 535-536. Abeles, F.B., et al. 1992, Ethylene in Plant Biology. Eds. Abeles, F.B., Morgan, P.W. and Saltveit, M.E. Academic Press, New York, pp 1-13. Abeles, F.B., et. al. 1992, Ethylene in Plant Biology. Eds. Abeles, F.B., Morgan, P.W. and Saltveit, M.E. Academic Press, New York, pp 285-291. Yang, S. F., et al, 1984, "Ethylene Biosynthesis and its Regulation in Higher Plants", Annu. Rev. Plant Physiol. 35:155-189. Nell, T.A., 1993, "Use and Care Advice" White J.W., ed., Geranium IV. The Grower's Manual, Edition Four, Ball Publishing, Geneva, IL pp 171-172. Chomczynski, P., et al, 1987, "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction", Analytical Biochemistry, 162, 156-159. Manning, K., 1991, "Isolation of Nucleic Acids from Plants by Differential Solvent Precipitation", Analytical Biochemistry 195, 45-50. Mullis, K.B., et al, 1987, "Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction", Methods in Enzymology, 155:335-350. Sanger, F., et al, 1977, "DNA sequencing with chain-terminating inhibitors", Proc. Natl. Acad. Sci. USA 74:5463-5467. Ranu, R.S., 1995, "DNA Sequencing with .tangle-solidup. Taq.RTM. Polymerase", Biotechniques 18:390-395. Ranu, R.S., et al, 1996, "In Vitro Translation of the Full-Length RNA Transcript of Figwort Mosaic Virus (Caulimovirus)", Gene Expression, vol. 5:143-153. Ranu, R.S., et al, 1979, "Regulation of Protein Synthesis in Rabbit Reticulocyte Lysates: Preparation of Efficient Protein Synthesis Lysates andthe Purification and Characterization of the Heme-Regulated Translational Inhibitory Protein Kinase", Methods in Enzymology vol. 60:459-484. Rottmann, W.H., et al, 1991, 1-Aminocyclopropane-1-Carboxylate Synthase in Tomato is Encoded by a Multigene Family Whose Transcription is Induced During Furit and Floral Senescence, Journal of Molecular Biology, 222, 937-961. Van Der Straeten, D., et al, 1990, "Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1-carboxylate synthase in tomato", Proceeding of the National Academy of Sciences, 87:4859-4863. Zarembinski, T.I., et al, 1994, "Ethylene biosynthesis and action: a case of conservation", Plant Molecular Biology, 26:1579-1597. Park, K.Y., et al, 1992, "Molecular cloning of an 1-aminocyclopropane-1-carboxylate synthase from senescing carnation flower petals", Plant Molecular Biology, 18:377-386. Schlagnhaufer, C.D., et al, 1995, "Molecular cloning of an ozone-induced 1-aminocyclopropane-1-carboxylate synthase cDNA and its relationship with a loss of rbcS in potato (Solanum tuberosum L.)", Plant Molecular Biology, 28:93-103. Wang, T.W. and Arteca, R.N., 1995, "Identification and Characterization of cDNAs Encoding Ethylene Biosynthetic Enzymes from Pelargonium x hortorum cv Snow Mass Leaves", Plant Physiology, 109:627-636. Sato, T., et al, 1989, "Cloning the mRNA encoding 1-aminocyclopropane-1-carboxylate synthase, the key enzyme for ethylene biosynthesis in plants", Proceedings of the National Academy of Sciences, 86:6621-6625. Nakajima, N., et al, 1990, "Molecular Cloning and Sequence of a Complementary DNA Encoding 1-Aminocyclopropane-1-carboxylate Synthase Induced by Tissue Wounding", Plant Cell Physiology, 31:1021-1029. Dong, J.G., et al, 1991, "Cloning of a cDNA encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its mRNA in ripening apple fruit", Planta, 185:38-45. Olson, D.C., et al, 1991, "Differential expression of two genes for 1-aminocyclopropane-1-carboxylate synthase in tomato fruits", Proceedings of National Academy of Sciences, 88:5340-5344. Smith, C.J.S., et al, 1991, " Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes", Nature, 334:724-726. Yip, W.K., et al, 1990, "Differential accumulation of transcripts for four tomato 1-aminocyclopropane-1-carboxylate synthase homologs under various conditions", Proceedings of the National Academy of Sciences, 89:2475-2479. Botella, J.R., et al, 1992, "Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid", Plant Molecular Biology, 20:425-436. Liang, X., et al, 1992, "The 1-aminocyclopropane-1-carboxylate synthase gene family of Arabidopsis thaliana", Proceedings of the National Academy of Sciences, 89:11046-11050. John, M.E., "An efficient method for isolation of RNA and DNA from plants containing polyphenolics", Nucleic Acids Research 20:2381, 1992. Logemann, J., et al, 1987, "Improved Method for the Isolation of RNA from Plant Tissues", Anal Biochem 163: 16-20. Kende, H., et al, 1993, "Ethylene Biosynthesis", Annual Review of Plant Physiology, Plant Mol. Biol., 44:283-307. Murray, A.J. 1993, "Expression of EFE Antisense RNA in Tomato Causes Retardation of Leaf Senescense and Most Fruit Ripening Characteristics", Cellular and Molecular Aspects of the Plant Hormone Ethylene, 327-328. Michael, M.Z., et al, 1993, "Cloning of Ethylene Biosynthetic Genes Involved in Petal Senescence of Carnation and Petunia, and their Antisense Expression in Transgenic Plants", Cellular and Molecular Aspects of the Plant Hormone Ethylene, 298-303. Gray, J.E., et al, 1993, "Altered Gene Expression, Leaf Senescence, and Fruit Ripening by Inhibiting Ethylene Synthesis with EFE-Antisense Genes", Cellular and Molecular Aspects of the Plant Hormone Ethylene, 82-89. Theologis, A., et al 1993, "Modifying Fruit Ripening By Supressing Gene Expression", Cellular and Molecular Aspects of the Plant Hormone Ethylene, 19-23. Hamilton, AJ et al 1990, "Antisense Gene that Inhibits Synthesis of the Hormone Ethylene in Transgenic Plants", Nature, 346:284-287. Karlin, Samuel and Altschul, Stephen, "Methods For Assessing The Statistical Significance Of Molecular Sequence Features By Using General Scoring Schemes", Proc. Natl. Acad. Sci. USA vol. 87, pp 2264-2268, Mar. 1990. Karlin, Samuel and Altschul, Stephen, "Applications and Statistcs For Multiple High-Scoring Segments In Molecular Sequences", Proc. Natl. Acad. Sci. USA vol. 90, pp 5873-5877, Mar. 1993. Altschul, Stephen F., et al, "Gapped BLAST and PSI-BLAST: A New Generation Of Protein Database Search Programs", Nucleic Acids Research, 1997, vol. 25, No. 17, pp. 3389-3402. Satot, Takahide, et al, "The 1-Aminocyclopropane-1-carboxylate Synthase of Cucurbita", The Journal of Biological Commistry, vol. 286, No. 6, Feb. 5, pp. 3752-3759 (1991). Altschul, S.F. (1997) "Sequence Comparison and Alignment," in "DNA and Protein Sequence Analysis" (M.J. Bishop & C. J. Rawlings, eds.) pp. 137-167, IRL Press, Oxford. T.F. Smith and M.S. Waterman, "Indentification of Common Molecular Subsequences," J. Molecular Biology 147:195-197 (1981). M.S. Waterman and M. Eggert, "A New Algorithm for Best Subsequence Alignments With Application to tRNA-rRNA Comparison," J. Molecular Biology 197:723-728 (1987). |
5'-GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3' [SEQ ID NO: 3] XhoI
5'AATTCGGCAGAG-3' [SEQ ID NO: 4] GCCGTCTCp5'