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United States Patent | 6,150,149 |
Kamada ,   et al. | November 21, 2000 |
The present invention provides: 1. A basidiomycete fungus-derived cytochrome P450 gene; 2. A vector comprising the cytochrome P450 of 1; 3. A transformant which is characterized in that the cytochrome P450 gene of 1 is introduced in a host cell; 4. A method for producing a cytochrome P450 comprising the steps of: incubating the transformant of 3; and the like.
Inventors: | Kamada; Takashi (Okayama, JP); Muraguchi; Hajime (Akita, JP) |
Assignee: | Sumitomo Chemical Company, Limited (Osaka, JP) |
Appl. No.: | 299662 |
Filed: | April 27, 1999 |
Nov 17, 1998[JP] | 10-327139 |
Current U.S. Class: | 435/189; 435/183; 435/252.3; 435/320.1; 536/23.2 |
Intern'l Class: | C12N 009/02; C12N 001/21; C12N 015/53 |
Field of Search: | 435/252.3,183,189,320.1 536/23.2 |
______________________________________ /500 mL Asparagine H.sub.2 O 1.14 g MgSO.sub.4 7H.sub.2 O 0.06 g Glucose 2.5 g Sucrose 85.6 g Dist. Water 430 mL 40*NCM salts 12.5 mL 1 mg/mL Thiamine 0.5 mL Agar 7.5 g Potato starch 2.5 g Autoclaved for 15 min 40*NCM salts: NH.sub.4 Cl 60 g Na.sub.2 HPO.sub.4 7H.sub.2 O 109.6 g KH.sub.2 PO.sub.4 54 g Na.sub.2 PO.sub.4 11.6 g Dist. Water 1 Liter sterilized by filtration ______________________________________
__________________________________________________________________________ # SEQUENCE LISTING - - - - <160> NUMBER OF SEQ ID NOS: 6 - - <210> SEQ ID NO 1 <211> LENGTH: 524 <212> TYPE: PRT <213> ORGANISM: Coprinus cinereus <220> FEATURE: <221> NAME/KEY: VARIANT <222> LOCATION: (26) <223> OTHER INFORMATION: Xaa=Leu or Phe - - <400> SEQUENCE: 1 - - Met Thr Thr Thr Ser Ser Ser Ile Phe Ala Gl - #y Leu Gly Cys Leu Leu 1 5 - # 10 - # 15 - - Val Ala Ser Ile Ile Tyr Phe Arg Lys Xaa Ly - #s Ala Asn Pro Ala Arg 20 - # 25 - # 30 - - Ala His Leu Pro Pro Gly Pro Lys Pro Ile Pr - #o Val Leu Gly Asn Val 35 - # 40 - # 45 - - Lys Asp Leu Arg Ala Lys Glu Leu Trp Leu Pr - #o Ala Met Asp Trp Ala 50 - # 55 - # 60 - - Lys Gln Tyr Gly Asp Ile Thr Tyr Leu His Va - #l Phe Gly Gln Gly Leu 65 - # 70 - # 75 - # 80 - - Thr Phe Ile Asn Ser Leu Glu Ser Ala Ser As - #p Leu Leu Glu Lys Arg 85 - # 90 - # 95 - - Gly Gly Met Tyr Ala Asp Lys Pro Gln Phe Th - #r Met Val Cys Glu Leu 100 - # 105 - # 110 - - Cys Asn Cys Lys Asn Met Val Ala Phe Thr Pr - #o Tyr Gly Glu Gln Ser 115 - # 120 - # 125 - - Lys Arg Gln Arg Arg Leu Met His Lys Ala Ph - #e Ala Pro Ala Arg Ile 130 - # 135 - # 140 - - Pro Asp Tyr His Pro Leu Met Glu Ser Ser Th - #r Asn Leu Phe Leu Arg 145 1 - #50 1 - #55 1 - #60 - - Asn Val Ile Ala Ser Pro Ala Asp Tyr Ile Gl - #y His Val Arg Arg Tyr 165 - # 170 - # 175 - - Ser Gly Ser Leu Thr Leu Asn Ile Val Tyr Gl - #y Tyr Glu Val Thr Ser 180 - # 185 - # 190 - - Asn Glu Asp Glu Tyr Leu Met Met Ala Glu Gl - #u Cys Val Gly Ile Leu 195 - # 200 - # 205 - - Ala Asn Glu Ile Ala Ser Ala Gly Gly Val Tr - #p Ala Val Asp Val Met 210 - # 215 - # 220 - - Pro Phe Leu Ala Lys Ile Pro Lys Trp Ala Gl - #u Gly Leu Pro Gly Met 225 2 - #30 2 - #35 2 - #40 - - Ser Phe Lys Arg Lys Ala Arg Lys Trp Lys Ly - #s Met Met Glu Asp Trp 245 - # 250 - # 255 - - Val Asp Gly Pro Phe Glu Tyr Val Lys Asn Th - #r Met Lys Ser Gly Thr 260 - # 265 - # 270 - - Tyr Lys Gln Ser Phe Cys Ser Ser Leu Leu As - #p Asp Glu Ser Ile Ser 275 - # 280 - # 285 - - Gln Thr Gln Glu His Phe Glu Phe Asp Leu Ly - #s Trp Thr Ala Asn Ser 290 - # 295 - # 300 - - Met Tyr Ala Ala Ser Ile Asp Thr Thr Ile Th - #r Ser Val Ala His Phe 305 3 - #10 3 - #15 3 - #20 - - Leu Leu Ala Met Met Lys His Pro Glu Val Le - #u Lys Lys Ala Gln His 325 - # 330 - # 335 - - Glu Ile Asp Thr Val Val Gly Gln Asp Arg Le - #u Pro Thr Phe Ser Asp 340 - # 345 - # 350 - - Arg Lys Ser Leu Pro Tyr Val Glu Ala Val Le - #u Ser Glu Thr Trp Arg 355 - # 360 - # 365 - - Trp Ala Ser Pro Val Pro Leu Ser Leu Pro Hi - #s Lys Leu Thr Glu Asp 370 - # 375 - # 380 - - Asp Val Tyr Arg Gly Met Tyr Ile Pro Lys Gl - #y Ser Leu Ile Phe Ala 385 3 - #90 3 - #95 4 - #00 - - Asn Ile Trp Ala Met Thr Arg Asp Glu Arg Il - #e Phe Pro Asp Pro Glu 405 - # 410 - # 415 - - Thr Phe Asn Pro Glu Arg Tyr Leu Asn Met As - #p Pro Glu Thr Lys Lys 420 - # 425 - # 430 - - Lys Gln Asp Pro Arg Asn Phe Ile Phe Gly Ph - #e Gly Arg Arg Leu Cys 435 - # 440 - # 445 - - Pro Gly Asn His Ile Val Asp Ala Ser Leu Tr - #p Leu Leu Val Val Arg 450 - # 455 - # 460 - - Met Met Ala Thr Leu Asp Ile Ser Thr Pro Va - #l Asp Glu Lys Gly Asn 465 4 - #70 4 - #75 4 - #80 - - Ala Ile Asp Ile Val Pro Val Phe Asp Asn Pr - #o Ile Phe Arg Thr Pro 485 - # 490 - # 495 - - Asn Pro Phe Pro Cys Asp Met Arg Pro Arg Se - #r Glu Lys Ala Val Asn 500 - # 505 - # 510 - - Leu Ile Arg Gln Tyr Ala Asp Pro Arg Ala Se - #r Ala 515 - # 520 - # 524 - - - - <210> SEQ ID NO 2 <211> LENGTH: 4364 <212> TYPE: DNA <213> ORGANISM: Coprinus cinereus <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (1229)..(1285) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (1575)..(1874) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2008)..(2063) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2083)..(2139) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2590)..(2639) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2772)..(2827) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2967)..(3015) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (3067)..(3120) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (3203)..(3254) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (3384)..(3441) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (3586)..(3641) - - <400> SEQUENCE: 2 - - atggccccga aaagaaattc tcgcgtgtca gtcgcccctg catacagaaa ga - #caaatgaa 60 - - tggattgagg tcgatgcggc gaggaatgaa cagacctagc atagggacga tc - #gtggcaga 120 - - ttggagagga aggttattcg ttgaacgtta cccgcctgct tccctttgaa at - #cggtttcc 180 - - gtagtgtcgg gatcggaacg agaggagaaa catcatgcga aagacgaaag aa - #ggtcgaca 240 - - ttccatcaca gcaatctgat accctcttgg ggagcaggcg acagaatctc gt - #cactgata 300 - - gagctcgaag ctacattgag atcggtgtta acgtcgatcc agagattgga ca - #gtcggagt 360 - - ctggcaagag agggcggtcc aagttcgaag aatttcgtat cggcgggcga ca - #atagggga 420 - - attatggaca gtgagcaggc caggttcgag ccatggtctg gagtgattca gc - #ccagataa 480 - - ccaagaagtc gagtatgggt ggttgtgggg gtagaaaggt gggagaacat ga - #ggactgtg 540 - - cagtcaggac ctgatgataa tgcgatagaa gccagttaag cattcggaca ag - #ggcgaact 600 - - ccagaacgtg cggtggttgt gctgcgtttc caggcggtgt gcagggtaaa cc - #gtgacacg 660 - - tcagagagag actgtgtgat taaagacatg gtcccatcag cttcgctgtc tc - #gacacaat 720 - - attgtgcttc tttttgcgcg gcgactgtga tctgtttcgt gaaaacagat tc - #ctgcaaaa 780 - - gacttttcat cttggcaagt ggaggatgac aggccagaaa gagcaacagc aa - #cggaaagg 840 - - ttggccgctc gatcttcgat ttggttccgt tggggccggt gcaacggggc ag - #cgccgtaa 900 - - gagaaattaa attagggtgg tgtttgtcca taacgggatt ccgtccgaat gg - #ggagaagg 960 - - ggtgttttct gtttccagat tgtgggtgcc gatggataaa tgggccaaat ct - #ggcgagat 1020 - - gggtgtttat gagactgggg ttgtcactga cataacttcg gcttctcgtc tt - #ctcagcca 1080 - - aaaaaattag cctttttctc aatacgcgtc aatccaaatc ctttaagacc ca - #gttaccgc 1140 - - ccctctcccc tattcgtttt ccccctcctc ccatttccca cccgtcgtcg ct - #ctcttccc 1200 - - tttcctcgct gccgagtcca tcttcaaggt acctatatca tatcttcctc gc - #ctaatagg 1260 - - agtcttctca tcgtcgtcga cgtagcgtat cacctcccgt tcccgtcatc aa - #cggctcag 1320 - - agaagagaac ttggtctata gcgttcattg ttccttcatc ttccctcaaa ct - # atg aca 1378 - # - # - # Met Thr - # - # - # 1 - - acg acg agc agt agt atc ttc gct ggt cta gg - #a tgc ctt ctt gtc gcc 1426 Thr Thr Ser Ser Ser Ile Phe Ala Gly Leu Gl - #y Cys Leu Leu Val Ala 5 - # 10 - # 15 - - tcc atc att tac ttc cgg aag ttt aaa gct aa - #c ccg gca cga gct cac 1474 Ser Ile Ile Tyr Phe Arg Lys Phe Lys Ala As - #n Pro Ala Arg Ala His 20 - # 25 - # 30 - - ctc cct cct ggt ccc aaa ccc att ccc gta ct - #c ggc aac gtc aaa gac 1522 Leu Pro Pro Gly Pro Lys Pro Ile Pro Val Le - #u Gly Asn Val Lys Asp 35 - # 40 - # 45 - # 50 - - ctt cga gcg aaa gag cta tgg ctg ccg gcg at - #g gac tgg gcg aag caa 1570 Leu Arg Ala Lys Glu Leu Trp Leu Pro Ala Me - #t Asp Trp Ala Lys Gln 55 - # 60 - # 65 - - tac g gtgagcctct tcaacttgtg ccttgggcga tatttcgttt ggaa - #tggcgg 1624 Tyr - - tgtaggagtt cgccgccatg gacgtcccgc aaagggtgga attaatgctc gc - #ctttgcaa 1684 - - atggcgagta gcttcaacgt gccataatgt acctcatacg agttatatag cc - #tcagtaat 1744 - - gctgagctaa ctcctcggtc gactccactg acgctttggc ttccgggcac ga - #ggctaggg 1804 - - gtggatacca acgtattctc gaagacgttc ctcgtttgag ccctatacta ac - #gcgctggg 1864 - - cttatactag gc gat atc acc tac ctt cac gtc ttt - # gga caa ggt ctc 1912 Gly Asp Ile Thr Tyr - #Leu His Val Phe Gly Gln Gly Leu - # 70 - # 75 - # 80 - - aca ttt atc aac tcc ttg gaa tct gca agc ga - #c ttg ctc gag aaa cga 1960 Thr Phe Ile Asn Ser Leu Glu Ser Ala Ser As - #p Leu Leu Glu Lys Arg 85 - # 90 - # 95 - - ggc ggc atg tac gcc gac aaa cct cag ttt ac - #c atg gtt tgc gaa ct g 2008 Gly Gly Met Tyr Ala Asp Lys Pro Gln Phe Th - #r Met Val Cys Glu Leu 100 - # 105 - # 110 - - taagtctttt tgtcgcttct ccactaccag gtgactgaac gcgaccggcc ct - #tag g tgc 2067 - # - # - # Cys - - aac tgt aag aat atg gtcagtcctt ctctaaccga caaagcgag - #c gatcctgagc 2122 Asn Cys Lys Asn Met 115 - - tctatccact tttgcag gtt gcc ttt acg ccc tac gga - #gaa cag tcg aag 2172 - # Val Ala Phe Thr Pro Tyr Gly Glu Gln Ser - #Lys - # 120 - # 125 - - cga caa cgg cga ttg atg cac aaa gct ttc gc - #c cct gct cgc atc ccg 2220 Arg Gln Arg Arg Leu Met His Lys Ala Phe Al - #a Pro Ala Arg Ile Pro 130 1 - #35 1 - #40 1 - #45 - - gat tat cat ccc tta atg gaa tca tca acc aa - #c ctc ttc ctc cga aac 2268 Asp Tyr His Pro Leu Met Glu Ser Ser Thr As - #n Leu Phe Leu Arg Asn 150 - # 155 - # 160 - - gtc att gca tcc ccg gcc gat tat atc ggt ca - #c gta cga agg tac tct 2316 Val Ile Ala Ser Pro Ala Asp Tyr Ile Gly Hi - #s Val Arg Arg Tyr Ser 165 - # 170 - # 175 - - ggt tca ctc acg ctc aac att gtc tat ggg ta - #c gaa gtc act tcc aac 2364 Gly Ser Leu Thr Leu Asn Ile Val Tyr Gly Ty - #r Glu Val Thr Ser Asn 180 - # 185 - # 190 - - gaa gac gaa tat ctt atg atg gcg gag gag tg - #t gtc ggt att ctc gct 2412 Glu Asp Glu Tyr Leu Met Met Ala Glu Glu Cy - #s Val Gly Ile Leu Ala 195 - # 200 - # 205 - - aac gaa att gcg agt gct gga ggt gtt tgg gc - #t gtc gat gtc atg cct 2460 Asn Glu Ile Ala Ser Ala Gly Gly Val Trp Al - #a Val Asp Val Met Pro 210 2 - #15 2 - #20 2 - #25 - - ttc ctc gct aag atc cct aaa tgg gca gag gg - #a ttg cca gga atg agc 2508 Phe Leu Ala Lys Ile Pro Lys Trp Ala Glu Gl - #y Leu Pro Gly Met Ser
230 - # 235 - # 240 - - ttt aaa cgg aag gcg agg aag tgg aag aag at - #g atg gag gat tgg gtt 2556 Phe Lys Arg Lys Ala Arg Lys Trp Lys Lys Me - #t Met Glu Asp Trp Val 245 - # 250 - # 255 - - gat gga cca ttc gaa tac gtc aag aac acc at - #g gtaagcttct ttcatatca a 2609 Asp Gly Pro Phe Glu Tyr Val Lys Asn Thr Me - #t 260 - # 265 - - ctcatcaacg tttaattaat tctccaacag aaa agc ggc act ta - #c aag caa tca 2663 - # Lys Ser - #Gly Thr Tyr Lys Gln Ser - # - # 270 - # 275 - - ttc tgc tct tcc cta tta gac gat gaa agt at - #c tcg caa acc cag gag 2711 Phe Cys Ser Ser Leu Leu Asp Asp Glu Ser Il - #e Ser Gln Thr Gln Glu 280 - # 285 - # 290 - - cac ttc gaa ttt gac ttg aag tgg acg gct aa - #c tcg atg tat gca gcc 2759 His Phe Glu Phe Asp Leu Lys Trp Thr Ala As - #n Ser Met Tyr Ala Ala 295 - # 300 - # 305 - - agc atc gat act gtgcgtgatc atacccgata tcaagttaat tg - #tcgcctga 2811 Ser Ile Asp Thr 310 - - ccccgccatc ttttag acg att acc tca gtc gct cac - #ttc cta ctt gca atg 2863 - #Thr Ile Thr Ser Val Ala His Phe Leu Leu A - #la Met - # 315 - # 320 - - atg aag cac ccc gaa gtc ttg aag aag gcg ca - #g cat gaa atc gat acc 2911 Met Lys His Pro Glu Val Leu Lys Lys Ala Gl - #n His Glu Ile Asp Thr 325 3 - #30 3 - #35 3 - #40 - - gtt gtc gga caa gat cgc ctc cct acc ttc ag - #c gac agg aag tct ttg 2959 Val Val Gly Gln Asp Arg Leu Pro Thr Phe Se - #r Asp Arg Lys Ser Leu 345 - # 350 - # 355 - - cca tat g gtacgttcca tgatagtccg actgaatgtc cacttatttg - #gtcgtgcag tt 3017 Pro Tyr - # - # - # Val - - gaa gcc gtc ctc tcc gag act tgg aga tgg gc - #t tcc ccc gtc cca ttg 3065 Glu Ala Val Leu Ser Glu Thr Trp Arg Trp Al - #a Ser Pro Val Pro Leu 360 3 - #65 3 - #70 3 - #75 - - a gtgagtagga tagtacctct ttgcacacgg ccaaactcag tcccacgac - #cttag gc 3122 - # - # - # Ser - - ttg cct cat aaa ttg act gaa gat gac gtg ta - #t cga gga atg tat atc 3170 Leu Pro His Lys Leu Thr Glu Asp Asp Val Ty - #r Arg Gly Met Tyr Ile 380 - # 385 - # 390 - - ccg aag ggt tcc ttg atc ttc gcc aat atc tg - # gtaagctcca ttgaactcca 3222 Pro Lys Gly Ser Leu Ile Phe Ala Asn Ile Tr - #p 395 - # 400 - - ggtttagcgg gccattctaa tcctctttga ag g gcg atg aca - #cga gat gag cgc 3276 - # - # Ala Met Thr Arg Asp Glu Arg - # - # 405 - # 410 - - atc ttc ccc gac ccg gag acg ttc aat ccc ga - #g aga tac ttg aac atg 3324 Ile Phe Pro Asp Pro Glu Thr Phe Asn Pro Gl - #u Arg Tyr Leu Asn Met 415 - # 420 - # 425 - - gat cca gag acc aag aag aag caa gat cca cg - #g aac ttc atc ttc gga 3372 Asp Pro Glu Thr Lys Lys Lys Gln Asp Pro Ar - #g Asn Phe Ile Phe Gly 430 - # 435 - # 440 - - ttc ggt cga ag gtttgtccac gcatgctggc tgcaactgga atc - #tgcaact 3423 Phe Gly Arg Arg 445 - - gactgtcatt cgccttag g ctt tgc cct gga aat cat - #atc gta gat gct tcg 3475 - # Leu Cys Pro Gly Asn His Ile Val - #Asp Ala Ser - # 450 - # 455 - - ctg tgg ctg ttg gtc gtt cgc atg atg gct ac - #c ctt gat atc tct acc 3523 Leu Trp Leu Leu Val Val Arg Met Met Ala Th - #r Leu Asp Ile Ser Thr 460 - # 465 - # 470 - - ccc gtc gac gag aaa ggc aat gca att gat at - #t gtt ccg gtt ttc gac 3571 Pro Val Asp Glu Lys Gly Asn Ala Ile Asp Il - #e Val Pro Val Phe Asp 475 - # 480 - # 485 - - aac cct atc ttc ag gttcgcccgc atccgtcccc tggttactct - # gtactgaatg 3625 Asn Pro Ile Phe Arg 490 - - tcttttctca tgctag a acg cca aat ccc ttc ccc t - #gc gat atg cga cca cgt 3678 - # Thr Pro Asn Pro Phe Pro Cys Asp Met - #Arg Pro Arg - # 495 - # 500 - # 505 - - tcc gag aag gct gta aat ctc att cga caa ta - #c gcc gac cct cgc gcc 3726 Ser Glu Lys Ala Val Asn Leu Ile Arg Gln Ty - #r Ala Asp Pro Arg Ala 510 - # 515 - # 520 - - tct gca taatcttctg tcagccatat acaaactacg acctcgctca ca - #gccgcgga 3782 Ser Ala - - ctcttcgaca ctggcctttt cgagcttata tgaccggaat tatcactcta gg - #gatctgtc 3842 - - caaaaggtta agcgacgatt gaatttttca gacatttttg catctcttcc ca - #cttctagc 3902 - - cttagattta aagttctacg gggcacagta tcacctccat cttttttctc aa - #ttatcctt 3962 - - gttcggtccc ggatggcaca ttctttatat cagccatggt cactcctttc tc - #tatctcgc 4022 - - tccctacatt tccacccatt tatcattatg acctgcattc atctcgaata cc - #cacaggat 4082 - - aggacggttc ggcgctttga ttaaattacg actcgcatct agcgtatact gt - #acaccttc 4142 - - attaccgtag tcgtcacctt tcaaccagaa acgcctcgaa acaccgcaca cc - #gcgtgtac 4202 - - tttcagtgtt aagtggaaat tcagatcttt ccaacctcgg gctttggatg tg - #atatttga 4262 - - atctcggaga cgatgaagcg agcggagact cctttgcgag gtttttggtg ct - #cgatgctt 4322 - - ctattcccgt tgctgccttt atcagttcca acttctgcgt ca - # - #4364 - - - - <210> SEQ ID NO 3 <211> LENGTH: 2236 <212> TYPE: DNA <213> ORGANISM: Coprinus cinereus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (162)..(1736) - - <400> SEQUENCE: 3 - - cgttttcccc ctcctcccat ttcccacccg tcgtcgctct cttccctttc ct - #cgctgccg 60 - - agtccatctt caagcgtatc acctcccgtt cccgtcatca acggcgcaga ga - #agagaact 120 - - tggtctatag cgttcattgt tccttcatct tccctcaaac t atg aca - #acg acg agc 176 - # - # Met Thr Thr Thr Ser - # - # 1 - # - - agt agt atc ttc gct ggt cta gga tgc ctt ct - #t gtc gcc tcc atc att 224 Ser Ser Ile Phe Ala Gly Leu Gly Cys Leu Le - #u Val Ala Ser Ile Ile 10 - # 15 - # 20 - - tac ttc cgg aag ctt aaa gct aac ccg gca cg - #a gct cac ctc cct cct 272 Tyr Phe Arg Lys Leu Lys Ala Asn Pro Ala Ar - #g Ala His Leu Pro Pro 25 - # 30 - # 35 - - ggt ccc aaa ccc att ccc gta ctc ggc aac gt - #c aaa gac ctt cga gcg 320 Gly Pro Lys Pro Ile Pro Val Leu Gly Asn Va - #l Lys Asp Leu Arg Ala 40 - # 45 - # 50 - - aaa gag cta tgg ctg ccg gcg atg gac tgg gc - #g aag caa tac ggc gat 368 Lys Glu Leu Trp Leu Pro Ala Met Asp Trp Al - #a Lys Gln Tyr Gly Asp 55 - # 60 - # 65 - - atc acc tac ctt cac gtc ttt gga caa ggt ct - #c aca ttt atc aac tcc 416 Ile Thr Tyr Leu His Val Phe Gly Gln Gly Le - #u Thr Phe Ile Asn Ser 70 - # 75 - # 80 - # 85 - - ttg gaa tct gca agc gac ttg ctc gag aaa cg - #a ggc ggc atg tac gcc 464 Leu Glu Ser Ala Ser Asp Leu Leu Glu Lys Ar - #g Gly Gly Met Tyr Ala 90 - # 95 - # 100 - - gac aaa cct cag ttt acc atg gtt tgc gaa ct - #g tgc aac tgt aag aat 512 Asp Lys Pro Gln Phe Thr Met Val Cys Glu Le - #u Cys Asn Cys Lys Asn 105 - # 110 - # 115 - - atg gtt gcc ttt acg ccc tac gga gaa cag tc - #g aag cga caa cgg cga 560 Met Val Ala Phe Thr Pro Tyr Gly Glu Gln Se - #r Lys Arg Gln Arg Arg 120 - # 125 - # 130 - - ttg atg cac aaa gct ttc gcc cct gct cgc at - #c ccg gat tat cat ccc 608 Leu Met His Lys Ala Phe Ala Pro Ala Arg Il - #e Pro Asp Tyr His Pro 135 - # 140 - # 145 - - tta atg gaa tca tca acc aac ctc ttc ctc cg - #a aac gtc att gca tcc 656 Leu Met Glu Ser Ser Thr Asn Leu Phe Leu Ar - #g Asn Val Ile Ala Ser 150 1 - #55 1 - #60 1 - #65 - - ccg gcc gat tat atc ggt cac gta cga agg ta - #c tct ggt tca ctc acg 704 Pro Ala Asp Tyr Ile Gly His Val Arg Arg Ty - #r Ser Gly Ser Leu Thr 170 - # 175 - # 180 - - ctc aac att gtc tat ggg tac gaa gtc act tc - #c aac gaa gac gaa tat 752 Leu Asn Ile Val Tyr Gly Tyr Glu Val Thr Se - #r Asn Glu Asp Glu Tyr 185 - # 190 - # 195 - - ctt atg atg gcg gag gag tgt gtc ggt att ct - #c gct aac gaa att gcg 800 Leu Met Met Ala Glu Glu Cys Val Gly Ile Le - #u Ala Asn Glu Ile Ala 200 - # 205 - # 210 - - agt gct gga ggt gtt tgg gct gtc gat gtc at - #g cct ttc ctc gct aag 848 Ser Ala Gly Gly Val Trp Ala Val Asp Val Me - #t Pro Phe Leu Ala Lys 215 - # 220 - # 225 - - atc cct aaa tgg gca gag gga ttg cca gga at - #g agc ttt aaa cgg aag 896 Ile Pro Lys Trp Ala Glu Gly Leu Pro Gly Me - #t Ser Phe Lys Arg Lys 230 2 - #35 2 - #40 2 - #45 - - gcg agg aag tgg aag aag atg atg gag gat tg - #g gtt gat gga cca ttc 944 Ala Arg Lys Trp Lys Lys Met Met Glu Asp Tr - #p Val Asp Gly Pro Phe 250 - # 255 - # 260 - - gaa tac gtc aag aac acc atg aaa agc ggc ac - #t tac aag caa tca ttc 992 Glu Tyr Val Lys Asn Thr Met Lys Ser Gly Th - #r Tyr Lys Gln Ser Phe 265 - # 270 - # 275 - - tgc tct tcc cta tta gac gat gaa agt atc tc - #g caa acc cag gag cac 1040 Cys Ser Ser Leu Leu Asp Asp Glu Ser Ile Se - #r Gln Thr Gln Glu His 280 - # 285 - # 290 - - ttc gaa ttt gac ttg aag tgg acg gct aac tc - #g atg tat gca gcc agc 1088 Phe Glu Phe Asp Leu Lys Trp Thr Ala Asn Se - #r Met Tyr Ala Ala Ser 295 - # 300 - # 305 - - atc gat act acg att acc tca gtc gct cac tt - #c cta ctt gca atg atg 1136 Ile Asp Thr Thr Ile Thr Ser Val Ala His Ph - #e Leu Leu Ala Met Met 310 3 - #15 3 - #20 3 - #25 - - aag cac ccc gaa gtc ttg aag aag gca cag ca - #t gaa atc gat acc gtt 1184 Lys His Pro Glu Val Leu Lys Lys Ala Gln Hi - #s Glu Ile Asp Thr Val 330 - # 335 - # 340 - - gtc gga caa gat cgc ctc ccc acc ttc agc ga - #c agg aag tct ttg cca 1232 Val Gly Gln Asp Arg Leu Pro Thr Phe Ser As - #p Arg Lys Ser Leu Pro 345 - # 350 - # 355 - - tat gtt gaa gcc gtc ctc tcc gag act tgg ag - #a tgg gct tcc ccc gtc 1280 Tyr Val Glu Ala Val Leu Ser Glu Thr Trp Ar - #g Trp Ala Ser Pro Val 360 - # 365 - # 370 - - cca ttg agc ttg cct cat aaa ttg act gaa ga - #t gac gtg tat cga gga 1328 Pro Leu Ser Leu Pro His Lys Leu Thr Glu As - #p Asp Val Tyr Arg Gly 375 - # 380 - # 385 - - atg tat atc ccg aag ggt tcc ttg atc ttc gc - #c aat atc tgg gcg atg 1376 Met Tyr Ile Pro Lys Gly Ser Leu Ile Phe Al - #a Asn Ile Trp Ala Met 390 3 - #95 4 - #00 4 - #05 - - aca cga gat gag cgc atc ttc ccc gac ccg ga - #g acg ttc aat ccc gag 1424 Thr Arg Asp Glu Arg Ile Phe Pro Asp Pro Gl - #u Thr Phe Asn Pro Glu 410 - # 415 - # 420 - - aga tac ttg aac atg gat cca gag acc aag aa - #g aag caa gat cca cgg 1472 Arg Tyr Leu Asn Met Asp Pro Glu Thr Lys Ly - #s Lys Gln Asp Pro Arg 425 - # 430 - # 435 - - aac ttc atc ttc gga ttc ggt cga agg ctt tg - #c cct gga aat cat atc 1520
Asn Phe Ile Phe Gly Phe Gly Arg Arg Leu Cy - #s Pro Gly Asn His Ile 440 - # 445 - # 450 - - gta gat gct tcg ctg tgg ctg ttg gtc gtt cg - #c atg atg gct acc ctt 1568 Val Asp Ala Ser Leu Trp Leu Leu Val Val Ar - #g Met Met Ala Thr Leu 455 - # 460 - # 465 - - gat atc tct acc ccc gtc gac gag aaa ggc aa - #t gca att gat att gtt 1616 Asp Ile Ser Thr Pro Val Asp Glu Lys Gly As - #n Ala Ile Asp Ile Val 470 4 - #75 4 - #80 4 - #85 - - ccg gtt ttc gac aac cct atc ttc aga acg cc - #a aat ccc ttc ccc tgc 1664 Pro Val Phe Asp Asn Pro Ile Phe Arg Thr Pr - #o Asn Pro Phe Pro Cys 490 - # 495 - # 500 - - gat atg cga cca cgt tcc gag aag gct gta aa - #t ctc att cga caa tac 1712 Asp Met Arg Pro Arg Ser Glu Lys Ala Val As - #n Leu Ile Arg Gln Tyr 505 - # 510 - # 515 - - gcc gac cct cgc gcc tct gca taatcttctg tcagccata - #t acaaactacg 1763 Ala Asp Pro Arg Ala Ser Ala 520 - - acctcgctca cagccgcgga ctcttcgaca ctggcctttt cgagcttata tg - #accggaat 1823 - - tatcactcta gggatctgtc caaaaggtta agcgacgatt gaatttttca ga - #catttttg 1883 - - catctcttcc cacttctagc cttagattta aagttctacg gggcacagta tc - #acctccat 1943 - - cttttttctc aattatcctt gttcggtccc ggatggcaca ttctttatat ca - #gccatggt 2003 - - cactcctttc tctatctcgc tccctacatt tccacccatt tatcattatg ac - #ctgcattc 2063 - - atctcgaata cccacaggat aggacggttc ggcgctttga ttaaattacg ac - #tcgcatct 2123 - - agcgtatact gtacaccttc attaccgtag tcgtcacctt tcaaccagaa ac - #gcctcgaa 2183 - - acaaaaaaaa aaaaaaaaaa aaaaaaaagt actctgcgtt gataccactg ct - #t 2236 - - - - <210> SEQ ID NO 4 <211> LENGTH: 506 <212> TYPE: PRT <213> ORGANISM: Coprinus cinereus <220> FEATURE: <221> NAME/KEY: VARIANT <222> LOCATION: (26) <223> OTHER INFORMATION: Xaa=Leu or Phe - - <400> SEQUENCE: 4 - - Met Thr Thr Thr Ser Ser Ser Ile Phe Ala Gl - #y Leu Gly Cys Leu Leu 1 5 - # 10 - # 15 - - Val Ala Ser Ile Ile Tyr Phe Arg Lys Xaa Ly - #s Ala Asn Pro Ala Arg 20 - # 25 - # 30 - - Ala His Leu Pro Pro Gly Pro Lys Pro Ile Pr - #o Val Leu Gly Asn Val 35 - # 40 - # 45 - - Lys Asp Leu Arg Ala Lys Glu Leu Trp Leu Pr - #o Ala Met Asp Trp Ala 50 - # 55 - # 60 - - Lys Gln Tyr Gly Asp Ile Thr Tyr Leu His Va - #l Phe Gly Gln Gly Leu 65 - # 70 - # 75 - # 80 - - Thr Phe Ile Asn Ser Leu Glu Ser Ala Ser As - #p Leu Leu Glu Lys Arg 85 - # 90 - # 95 - - Gly Gly Met Tyr Ala Asp Lys Pro Gln Phe Th - #r Met Val Cys Glu Leu 100 - # 105 - # 110 - - Cys Asn Cys Lys Asn Met Val Ala Phe Thr Pr - #o Tyr Gly Glu Gln Ser 115 - # 120 - # 125 - - Lys Arg Gln Arg Arg Leu Met His Lys Ala Ph - #e Ala Pro Ala Arg Ile 130 - # 135 - # 140 - - Pro Asp Tyr His Pro Leu Met Glu Ser Ser Th - #r Asn Leu Phe Leu Arg 145 1 - #50 1 - #55 1 - #60 - - Asn Val Ile Ala Ser Pro Ala Asp Tyr Ile Gl - #y His Val Arg Arg Tyr 165 - # 170 - # 175 - - Ser Gly Ser Leu Thr Leu Asn Ile Val Tyr Gl - #y Tyr Glu Val Thr Ser 180 - # 185 - # 190 - - Asn Glu Asp Glu Tyr Leu Met Met Ala Glu Gl - #u Cys Val Gly Ile Leu 195 - # 200 - # 205 - - Ala Asn Glu Ile Ala Ser Ala Gly Gly Val Tr - #p Ala Val Asp Val Met 210 - # 215 - # 220 - - Pro Phe Leu Ala Lys Ile Pro Lys Trp Ala Gl - #u Gly Leu Pro Gly Met 225 2 - #30 2 - #35 2 - #40 - - Ser Phe Lys Arg Lys Ala Arg Lys Trp Lys Ly - #s Met Met Glu Asp Trp 245 - # 250 - # 255 - - Val Asp Gly Pro Phe Glu Tyr Val Lys Asn Th - #r Met Lys Ser Gly Thr 260 - # 265 - # 270 - - Tyr Lys Gln Ser Phe Cys Ser Ser Leu Leu As - #p Asp Glu Ser Ile Ser 275 - # 280 - # 285 - - Gln Thr Gln Glu His Phe Glu Phe Asp Leu Ly - #s Trp Thr Ala Asn Ser 290 - # 295 - # 300 - - Met Tyr Ala Ala Ser Ile Asp Thr Thr Ile Th - #r Ser Val Ala His Phe 305 3 - #10 3 - #15 3 - #20 - - Leu Leu Ala Met Met Lys His Pro Glu Val Le - #u Lys Lys Ala Gln His 325 - # 330 - # 335 - - Glu Ile Asp Thr Val Val Gly Gln Asp Arg Le - #u Pro Thr Phe Ser Asp 340 - # 345 - # 350 - - Arg Lys Ser Leu Pro Tyr Val Glu Ala Val Le - #u Ser Glu Thr Trp Arg 355 - # 360 - # 365 - - Trp Ala Ser Pro Val Pro Leu Ser Leu Pro Hi - #s Lys Leu Thr Glu Asp 370 - # 375 - # 380 - - Asp Val Tyr Arg Gly Met Tyr Ile Pro Lys Gl - #y Ser Leu Ile Phe Ala 385 3 - #90 3 - #95 4 - #00 - - Asn Ile Trp Ala Met Thr Arg Asp Glu Arg Il - #e Phe Pro Asp Pro Glu 405 - # 410 - # 415 - - Thr Phe Asn Pro Glu Arg Tyr Leu Asn Met As - #p Pro Glu Thr Lys Lys 420 - # 425 - # 430 - - Lys Gln Asp Pro Arg Asn Phe Ile Phe Gly Ph - #e Gly Arg Arg Leu Cys 435 - # 440 - # 445 - - Pro Gly Asn His Ile Val Asp Ala Ser Leu Tr - #p Leu Leu Val Val Arg 450 - # 455 - # 460 - - Met Met Ala Thr Leu Asp Ile Ser Thr Pro Va - #l Asp Glu Lys Gly Asn 465 4 - #70 4 - #75 4 - #80 - - Ala Ile Asp Ile Val Pro Val Phe Asp Asn Pr - #o Ile Phe Arg Thr Pro 485 - # 490 - # 495 - - Asn Pro Phe Pro Cys Asp Met Arg Pro Arg 500 - # 505 506 - - - - <210> SEQ ID NO 5 <211> LENGTH: 4360 <212> TYPE: DNA <213> ORGANISM: Coprinus cinereus <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (1229)..(1285) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (1575)..(1874) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2008)..(2063) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2083)..(2139) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2590)..(2639) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2772)..(2827) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (2967)..(3015) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (3067)..(3120) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (3203)..(3254) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (3384)..(3441) <220> FEATURE: <221> NAME/KEY: intron <222> LOCATION: (3586)..(3641) - - <400> SEQUENCE: 5 - - atggccccga aaagaaattc tcgcgtgtca gtcgcccctg catacagaaa ga - #caaatgaa 60 - - tggattgagg tcgatgcggc gaggaatgaa cagacctagc atagggacga tc - #gtggcaga 120 - - ttggagagga aggttattcg ttgaacgtta cccgcctgct tccctttgaa at - #cggtttcc 180 - - gtagtgtcgg gatcggaacg agaggagaaa catcatgcga aagacgaaag aa - #ggtcgaca 240 - - ttccatcaca gcaatctgat accctcttgg ggagcaggcg acagaatctc gt - #cactgata 300 - - gagctcgaag ctacattgag atcggtgtta acgtcgatcc agagattgga ca - #gtcggagt 360 - - ctggcaagag agggcggtcc aagttcgaag aatttcgtat cggcgggcga ca - #atagggga 420 - - attatggaca gtgagcaggc caggttcgag ccatggtctg gagtgattca gc - #ccagataa 480 - - ccaagaagtc gagtatgggt ggttgtgggg gtagaaaggt gggagaacat ga - #ggactgtg 540 - - cagtcaggac ctgatgataa tgcgatagaa gccagttaag cattcggaca ag - #ggcgaact 600 - - ccagaacgtg cggtggttgt gctgcgtttc caggcggtgt gcagggtaaa cc - #gtgacacg 660 - - tcagagagag actgtgtgat taaagacatg gtcccatcag cttcgctgtc tc - #gacacaat 720 - - attgtgcttc tttttgcgcg gcgactgtga tctgtttcgt gaaaacagat tc - #ctgcaaaa 780 - - gacttttcat cttggcaagt ggaggatgac aggccagaaa gagcaacagc aa - #cggaaagg 840 - - ttggccgctc gatcttcgat ttggttccgt tggggccggt gcaacggggc ag - #cgccgtaa 900 - - gagaaattaa attagggtgg tgtttgtcca taacgggatt ccgtccgaat gg - #ggagaagg 960 - - ggtgttttct gtttccagat tgtgggtgcc gatggataaa tgggccaaat ct - #ggcgagat 1020 - - gggtgtttat gagactgggg ttgtcactga cataacttcg gcttctcgtc tt - #ctcagcca 1080 - - aaaaaattag cctttttctc aatacgcgtc aatccaaatc ctttaagacc ca - #gttaccgc 1140 - - ccctctcccc tattcgtttt ccccctcctc ccatttccca cccgtcgtcg ct - #ctcttccc 1200 - - tttcctcgct gccgagtcca tcttcaaggt acctatatca tatcttcctc gc - #ctaatagg 1260 - - agtcttctca tcgtcgtcga cgtagcgtat cacctcccgt tcccgtcatc aa - #cggctcag 1320 - - agaagagaac ttggtctata gcgttcattg ttccttcatc ttccctcaaa ct - # atg aca 1378 - # - # - # Met Thr - # - # - # 1 - - acg acg agc agt agt atc ttc gct ggt cta gg - #a tgc ctt ctt gtc gcc 1426 Thr Thr Ser Ser Ser Ile Phe Ala Gly Leu Gl - #y Cys Leu Leu Val Ala 5 - # 10 - # 15 - - tcc atc att tac ttc cgg aag ttt aaa gct aa - #c ccg gca cga gct cac 1474 Ser Ile Ile Tyr Phe Arg Lys Phe Lys Ala As - #n Pro Ala Arg Ala His 20 - # 25 - # 30 - - ctc cct cct ggt ccc aaa ccc att ccc gta ct - #c ggc aac gtc aaa gac 1522 Leu Pro Pro Gly Pro Lys Pro Ile Pro Val Le - #u Gly Asn Val Lys Asp 35 - # 40 - # 45 - # 50 - - ctt cga gcg aaa gag cta tgg ctg ccg gcg at - #g gac tgg gcg aag caa 1570 Leu Arg Ala Lys Glu Leu Trp Leu Pro Ala Me - #t Asp Trp Ala Lys Gln 55 - # 60 - # 65 - - tac g gtgagcctct tcaacttgtg ccttgggcga tatttcgttt ggaa - #tggcgg 1624 Tyr - - tgtaggagtt cgccgccatg gacgtcccgc aaagggtgga attaatgctc gc - #ctttgcaa 1684 - - atggcgagta gcttcaacgt gccataatgt acctcatacg agttatatag cc - #tcagtaat 1744 - - gctgagctaa ctcctcggtc gactccactg acgctttggc ttccgggcac ga - #ggctaggg 1804 - - gtggatacca acgtattctc gaagacgttc ctcgtttgag ccctatacta ac - #gcgctggg 1864 - - cttatacta g gc gat atc acc tac ctt cac gtc - #ttt gga caa ggt ctc 1912 Gly Asp Ile Thr - #Tyr Leu His Val Phe Gly Gln Gly Leu - # 70 - # 75 - # 80 - - aca ttt atc aac tcc ttg gaa tct gca agc ga - #c ttg ctc gag aaa cga 1960 Thr Phe Ile Asn Ser Leu Glu Ser Ala Ser As - #p Leu Leu Glu Lys Arg 85 - # 90 - # 95 - - ggc ggc atg tac gcc gac aaa cct cag ttt ac - #c atg gtt tgc gaa ct g 2008 Gly Gly Met Tyr Ala Asp Lys Pro Gln Phe Th - #r Met Val Cys Glu Leu 100 - # 105 - # 110 - - taagtctttt tgtcgcttct ccactaccag gtgactgaac gcgaccggcc ct - #tag g tgc 2067 - # - # - # Cys - - aac tgt aag aat atg gtcagtcctt ctctaaccga caaagcgag - #c gatcctgagc 2122 Asn Cys Lys Asn Met 115
- - tctatccact tttgcag gtt gcc ttt acg ccc tac gga - #gaa cag tcg aag 2172 - # Val Ala Phe Thr Pro Tyr Gly Glu Gln Ser - #Lys - # 120 - # 125 - - cga caa cgg cga ttg atg cac aaa gct ttc gc - #c cct gct cgc atc ccg 2220 Arg Gln Arg Arg Leu Met His Lys Ala Phe Al - #a Pro Ala Arg Ile Pro 130 1 - #35 1 - #40 1 - #45 - - gat tat cat ccc tta atg gaa tca tca acc aa - #c ctc ttc ctc cga aac 2268 Asp Tyr His Pro Leu Met Glu Ser Ser Thr As - #n Leu Phe Leu Arg Asn 150 - # 155 - # 160 - - gtc att gca tcc ccg gcc gat tat atc ggt ca - #c gta cga agg tac tct 2316 Val Ile Ala Ser Pro Ala Asp Tyr Ile Gly Hi - #s Val Arg Arg Tyr Ser 165 - # 170 - # 175 - - ggt tca ctc acg ctc aac att gtc tat ggg ta - #c gaa gtc act tcc aac 2364 Gly Ser Leu Thr Leu Asn Ile Val Tyr Gly Ty - #r Glu Val Thr Ser Asn 180 - # 185 - # 190 - - gaa gac gaa tat ctt atg atg gcg gag gag tg - #t gtc ggt att ctc gct 2412 Glu Asp Glu Tyr Leu Met Met Ala Glu Glu Cy - #s Val Gly Ile Leu Ala 195 - # 200 - # 205 - - aac gaa att gcg agt gct gga ggt gtt tgg gc - #t gtc gat gtc atg cct 2460 Asn Glu Ile Ala Ser Ala Gly Gly Val Trp Al - #a Val Asp Val Met Pro 210 2 - #15 2 - #20 2 - #25 - - ttc ctc gct aag atc cct aaa tgg gca gag gg - #a ttg cca gga atg agc 2508 Phe Leu Ala Lys Ile Pro Lys Trp Ala Glu Gl - #y Leu Pro Gly Met Ser 230 - # 235 - # 240 - - ttt aaa cgg aag gcg agg aag tgg aag aag at - #g atg gag gat tgg gtt 2556 Phe Lys Arg Lys Ala Arg Lys Trp Lys Lys Me - #t Met Glu Asp Trp Val 245 - # 250 - # 255 - - gat gga cca ttc gaa tac gtc aag aac acc at - #g gtaagcttct ttcatatca a 2609 Asp Gly Pro Phe Glu Tyr Val Lys Asn Thr Me - #t 260 - # 265 - - ctcatcaacg tttaattaat tctccaacag aaa agc ggc act ta - #c aag caa tca 2663 - # Lys Ser - #Gly Thr Tyr Lys Gln Ser - # - # 270 - # 275 - - ttc tgc tct tcc cta tta gac gat gaa agt at - #c tcg caa acc cag gag 2711 Phe Cys Ser Ser Leu Leu Asp Asp Glu Ser Il - #e Ser Gln Thr Gln Glu 280 - # 285 - # 290 - - cac ttc gaa ttt gac ttg aag tgg acg gct aa - #c tcg atg tat gca gcc 2759 His Phe Glu Phe Asp Leu Lys Trp Thr Ala As - #n Ser Met Tyr Ala Ala 295 - # 300 - # 305 - - agc atc gat act gtgcgtgatc atacccgata tcaagttaat tg - #tcgcctga 2811 Ser Ile Asp Thr 310 - - ccccgccatc ttttag acg att acc tca gtc gct cac - #ttc cta ctt gca atg 2863 - #Thr Ile Thr Ser Val Ala His Phe Leu Leu A - #la Met - # 315 - # 320 - - atg aag cac ccc gaa gtc ttg aag aag gcg ca - #g cat gaa atc gat acc 2911 Met Lys His Pro Glu Val Leu Lys Lys Ala Gl - #n His Glu Ile Asp Thr 325 3 - #30 3 - #35 3 - #40 - - gtt gtc gga caa gat cgc ctc cct acc ttc ag - #c gac agg aag tct ttg 2959 Val Val Gly Gln Asp Arg Leu Pro Thr Phe Se - #r Asp Arg Lys Ser Leu 345 - # 350 - # 355 - - cca tat g gtacgttcca tgatagtccg actgaatgtc cacttatttg - #gtcgtgca g tt 3017 Pro Tyr - # - # - # Val - - gaa gcc gtc ctc tcc gag act tgg aga tgg gc - #t tcc ccc gtc cca ttg 3065 Glu Ala Val Leu Ser Glu Thr Trp Arg Trp Al - #a Ser Pro Val Pro Leu 360 3 - #65 3 - #70 3 - #75 - - a gtgagtagga tagtacctct ttgcacacgg ccaaactcag tcccacgacc - #ttag gc 3122 - # - # - # Ser - - ttg cct cat aaa ttg act gaa gat gac gtg ta - #t cga gga atg tat atc 3170 Leu Pro His Lys Leu Thr Glu Asp Asp Val Ty - #r Arg Gly Met Tyr Ile 380 - # 385 - # 390 - - ccg aag ggt tcc ttg atc ttc gcc aat atc tg - # g taagctccat tgaactccag 3223 Pro Lys Gly Ser Leu Ile Phe Ala Asn Ile Tr - #p 395 - # 400 - - gtttagcggg ccattctaat cctctttgaa g g gcg atg aca - #cga gat gag cgc 3276 - # - # Ala Met Thr Arg Asp Glu Arg - # - # 405 - # 410 - - atc ttc ccc gac ccg gag acg ttc aat ccc ga - #g aga tac ttg aac atg 3324 Ile Phe Pro Asp Pro Glu Thr Phe Asn Pro Gl - #u Arg Tyr Leu Asn Met 415 - # 420 - # 425 - - gat cca gag acc aag aag aag caa gat cca cg - #g aac ttc atc ttc gga 3372 Asp Pro Glu Thr Lys Lys Lys Gln Asp Pro Ar - #g Asn Phe Ile Phe Gly 430 - # 435 - # 440 - - ttc ggt cga ag gtttgtccac gcatgctggc tgcaactgga atc - #tgcaact 3423 Phe Gly Arg Arg 445 - - gactgtcatt cgccttag g ctt tgc cct gga aat cat - #atc gta gat gct tcg 3475 - # Leu Cys Pro Gly Asn His Ile Val - #Asp Ala Ser - # 450 - # 455 - - ctg tgg ctg ttg gtc gtt cgc atg atg gct ac - #c ctt gat atc tct acc 3523 Leu Trp Leu Leu Val Val Arg Met Met Ala Th - #r Leu Asp Ile Ser Thr 460 - # 465 - # 470 - - ccc gtc gac gag aaa ggc aat gca att gat at - #t gtt ccg gtt ttc gac 3571 Pro Val Asp Glu Lys Gly Asn Ala Ile Asp Il - #e Val Pro Val Phe Asp 475 - # 480 - # 485 - - aac cct atc ttc ag gttcgcccgc atccgtcccc tggttactct - # gtactgaatg 3625 Asn Pro Ile Phe Arg 490 - - tcttttctca tgctag a acg cca aat ccc ttc ccc t - #gc gat atg cga cca cgt 3678 - # Thr Pro Asn Pro Phe Pro Cys Asp Met - #Arg Pro Arg - # 495 - # 500 - # 505 - - tgaaggctgt aaatctcatt cgacaatacg ccgaccctcg cgcctctgca ta - #atcttctg 3738 - - tcagccatat acaaactacg acctcgctca cagccgcgga ctcttcgaca ct - #ggcctttt 3798 - - cgagcttata tgaccggaat tatcactcta gggatctgtc caaaaggtta ag - #cgacgatt 3858 - - gaatttttca gacatttttg catctcttcc cacttctagc cttagattta aa - #gttctacg 3918 - - gggcacagta tcacctccat cttttttctc aattatcctt gttcggtccc gg - #atggcaca 3978 - - ttctttatat cagccatggt cactcctttc tctatctcgc tccctacatt tc - #cacccatt 4038 - - tatcattatg acctgcattc atctcgaata cccacaggat aggacggttc gg - #cgctttga 4098 - - ttaaattacg actcgcatct agcgtatact gtacaccttc attaccgtag tc - #gtcacctt 4158 - - tcaaccagaa acgcctcgaa acaccgcaca ccgcgtgtac tttcagtgtt aa - #gtggaaat 4218 - - tcagatcttt ccaacctcgg gctttggatg tgatatttga atctcggaga cg - #atgaagcg 4278 - - agcggagact cctttgcgag gtttttggtg ctcgatgctt ctattcccgt tg - #ctgccttt 4338 - - atcagttcca acttctgcgt ca - # - # 4360 - - - - <210> SEQ ID NO 6 <211> LENGTH: 1521 <212> TYPE: DNA <213> ORGANISM: Coprinus cinereus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(1521) - - <400> SEQUENCE: 6 - - atg aca acg acg agc agt agt atc ttc gct gg - #t cta gga tgc ctt ctt 48 Met Thr Thr Thr Ser Ser Ser Ile Phe Ala Gl - #y Leu Gly Cys Leu Leu 1 5 - # 10 - # 15 - - gtc gcc tcc atc att tac ttc cgg aag ctt aa - #a gct aac ccg gca cga 96 Val Ala Ser Ile Ile Tyr Phe Arg Lys Leu Ly - #s Ala Asn Pro Ala Arg 20 - # 25 - # 30 - - gct cac ctc cct cct ggt ccc aaa ccc att cc - #c gta ctc ggc aac gtc 144 Ala His Leu Pro Pro Gly Pro Lys Pro Ile Pr - #o Val Leu Gly Asn Val 35 - # 40 - # 45 - - aaa gac ctt cga gcg aaa gag cta tgg ctg cc - #g gcg atg gac tgg gcg 192 Lys Asp Leu Arg Ala Lys Glu Leu Trp Leu Pr - #o Ala Met Asp Trp Ala 50 - # 55 - # 60 - - aag caa tac ggc gat atc acc tac ctt cac gt - #c ttt gga caa ggt ctc 240 Lys Gln Tyr Gly Asp Ile Thr Tyr Leu His Va - #l Phe Gly Gln Gly Leu 65 - # 70 - # 75 - # 80 - - aca ttt atc aac tcc ttg gaa tct gca agc ga - #c ttg ctc gag aaa cga 288 Thr Phe Ile Asn Ser Leu Glu Ser Ala Ser As - #p Leu Leu Glu Lys Arg 85 - # 90 - # 95 - - ggc ggc atg tac gcc gac aaa cct cag ttt ac - #c atg gtt tgc gaa ctg 336 Gly Gly Met Tyr Ala Asp Lys Pro Gln Phe Th - #r Met Val Cys Glu Leu 100 - # 105 - # 110 - - tgc aac tgt aag aat atg gtt gcc ttt acg cc - #c tac gga gaa cag tcg 384 Cys Asn Cys Lys Asn Met Val Ala Phe Thr Pr - #o Tyr Gly Glu Gln Ser 115 - # 120 - # 125 - - aag cga caa cgg cga ttg atg cac aaa gct tt - #c gcc cct gct cgc atc 432 Lys Arg Gln Arg Arg Leu Met His Lys Ala Ph - #e Ala Pro Ala Arg Ile 130 - # 135 - # 140 - - ccg gat tat cat ccc tta atg gaa tca tca ac - #c aac ctc ttc ctc cga 480 Pro Asp Tyr His Pro Leu Met Glu Ser Ser Th - #r Asn Leu Phe Leu Arg 145 1 - #50 1 - #55 1 - #60 - - aac gtc att gca tcc ccg gcc gat tat atc gg - #t cac gta cga agg tac 528 Asn Val Ile Ala Ser Pro Ala Asp Tyr Ile Gl - #y His Val Arg Arg Tyr 165 - # 170 - # 175 - - tct ggt tca ctc acg ctc aac att gtc tat gg - #g tac gaa gtc act tcc 576 Ser Gly Ser Leu Thr Leu Asn Ile Val Tyr Gl - #y Tyr Glu Val Thr Ser 180 - # 185 - # 190 - - aac gaa gac gaa tat ctt atg atg gcg gag ga - #g tgt gtc ggt att ctc 624 Asn Glu Asp Glu Tyr Leu Met Met Ala Glu Gl - #u Cys Val Gly Ile Leu 195 - # 200 - # 205 - - gct aac gaa att gcg agt gct gga ggt gtt tg - #g gct gtc gat gtc atg 672 Ala Asn Glu Ile Ala Ser Ala Gly Gly Val Tr - #p Ala Val Asp Val Met 210 - # 215 - # 220 - - cct ttc ctc gct aag atc cct aaa tgg gca ga - #g gga ttg cca gga atg 720 Pro Phe Leu Ala Lys Ile Pro Lys Trp Ala Gl - #u Gly Leu Pro Gly Met 225 2 - #30 2 - #35 2 - #40 - - agc ttt aaa cgg aag gcg agg aag tgg aag aa - #g atg atg gag gat tgg 768 Ser Phe Lys Arg Lys Ala Arg Lys Trp Lys Ly - #s Met Met Glu Asp Trp 245 - # 250 - # 255 - - gtt gat gga cca ttc gaa tac gtc aag aac ac - #c atg aaa agc ggc act 816 Val Asp Gly Pro Phe Glu Tyr Val Lys Asn Th - #r Met Lys Ser Gly Thr 260 - # 265 - # 270 - - tac aag caa tca ttc tgc tct tcc cta tta ga - #c gat gaa agt atc tcg 864 Tyr Lys Gln Ser Phe Cys Ser Ser Leu Leu As - #p Asp Glu Ser Ile Ser 275 - # 280 - # 285 - - caa acc cag gag cac ttc gaa ttt gac ttg aa - #g tgg acg gct aac tcg 912 Gln Thr Gln Glu His Phe Glu Phe Asp Leu Ly - #s Trp Thr Ala Asn Ser 290 - # 295 - # 300 - - atg tat gca gcc agc atc gat act acg att ac - #c tca gtc gct cac ttc 960 Met Tyr Ala Ala Ser Ile Asp Thr Thr Ile Th - #r Ser Val Ala His Phe 305 3 - #10 3 - #15 3 - #20 - - cta ctt gca atg atg aag cac ccc gaa gtc tt - #g aag aag gca cag cat 1008 Leu Leu Ala Met Met Lys His Pro Glu Val Le - #u Lys Lys Ala Gln His 325 - # 330 - # 335 - - gaa atc gat acc gtt gtc gga caa gat cgc ct - #c ccc acc ttc agc gac 1056 Glu Ile Asp Thr Val Val Gly Gln Asp Arg Le - #u Pro Thr Phe Ser Asp 340 - # 345 - # 350 - - agg aag tct ttg cca tat gtt gaa gcc gtc ct - #c tcc gag act tgg aga
1104 Arg Lys Ser Leu Pro Tyr Val Glu Ala Val Le - #u Ser Glu Thr Trp Arg 355 - # 360 - # 365 - - tgg gct tcc ccc gtc cca ttg agc ttg cct ca - #t aaa ttg act gaa gat 1152 Trp Ala Ser Pro Val Pro Leu Ser Leu Pro Hi - #s Lys Leu Thr Glu Asp 370 - # 375 - # 380 - - gac gtg tat cga gga atg tat atc ccg aag gg - #t tcc ttg atc ttc gcc 1200 Asp Val Tyr Arg Gly Met Tyr Ile Pro Lys Gl - #y Ser Leu Ile Phe Ala 385 3 - #90 3 - #95 4 - #00 - - aat atc tgg gcg atg aca cga gat gag cgc at - #c ttc ccc gac ccg gag 1248 Asn Ile Trp Ala Met Thr Arg Asp Glu Arg Il - #e Phe Pro Asp Pro Glu 405 - # 410 - # 415 - - acg ttc aat ccc gag aga tac ttg aac atg ga - #t cca gag acc aag aag 1296 Thr Phe Asn Pro Glu Arg Tyr Leu Asn Met As - #p Pro Glu Thr Lys Lys 420 - # 425 - # 430 - - aag caa gat cca cgg aac ttc atc ttc gga tt - #c ggt cga agg ctt tgc 1344 Lys Gln Asp Pro Arg Asn Phe Ile Phe Gly Ph - #e Gly Arg Arg Leu Cys 435 - # 440 - # 445 - - cct gga aat cat atc gta gat gct tcg ctg tg - #g ctg ttg gtc gtt cgc 1392 Pro Gly Asn His Ile Val Asp Ala Ser Leu Tr - #p Leu Leu Val Val Arg 450 - # 455 - # 460 - - atg atg gct acc ctt gat atc tct acc ccc gt - #c gac gag aaa ggc aat 1440 Met Met Ala Thr Leu Asp Ile Ser Thr Pro Va - #l Asp Glu Lys Gly Asn 465 4 - #70 4 - #75 4 - #80 - - gca att gat att gtt ccg gtt ttc gac aac cc - #t atc ttc aga acg cca 1488 Ala Ile Asp Ile Val Pro Val Phe Asp Asn Pr - #o Ile Phe Arg Thr Pro 485 - # 490 - # 495 - - aat ccc ttc ccc tgc gat atg cga cca cgt tg - #a - # 1521 Asn Pro Phe Pro Cys Asp Met Arg Pro Arg 500 - # 505 __________________________________________________________________________