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United States Patent | 6,063,601 |
Herrera-Estrella ,   et al. | May 16, 2000 |
Chimaeric DNA sequence which encodes: 1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or polypeptide of a plant and 2) a protein or polypeptide that is heterologous to the transit peptide. The chimaeric DNA sequence can be used as a vector for transforming a plant cell so that a chimaeric precursor of the heterologous protein or polypeptide is produced in the cytoplasm of the cell and the chimaeric precursor then transports the heterologous protein or polypeptide in vivo into a chloroplast of the cell.
Inventors: | Herrera-Estrella; Luis (Ghent, BE); Van Den Broeck; Guidi (Ghent, BE); Van Montagu; Marc (Brussels, BE); Schreier; Peter (Cologne, DE); Schell; Jeff (Cologne, DE); Bohnert; Hans J. (Tucson, AZ); Cash more; Anthony R. (Woodside, NY); Timko; Michael P. (New York, NY); Kausch; Albert P. (Durham, NH) |
Assignee: | Plant Genetic Systems, N.V. (Brussels, BE); Bayer A.G. (Lever Kusen, DE) |
Appl. No.: | 468317 |
Filed: | June 6, 1995 |
Dec 28, 1984[GB] | 8432757 | |
Jan 07, 1985[GB] | 850036 |
Current U.S. Class: | 800/278; 435/69.8; 435/70.1; 435/419; 536/23.4; 536/23.6; 536/23.7; 536/24.1; 800/288; 800/294 |
Intern'l Class: | C12N 015/29; C12N 015/62; C12N 015/82; A01H 005/00 |
Field of Search: | 435/69.7,69.8,70.1,172.3,419 536/23.4,24.1,23.6,23.7 800/205 |
Foreign Patent Documents | |||
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0 356 061 | Feb., 1990 | EP. | |
84/02913 | Aug., 1984 | WO. |
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M A S M I S S S A V T T ATG GCT TCT ATG ATA TCC TCT TCC GCT GTG ACA ACA - V S R A S R G Q S A A V GTC AGC CCT GCC TCT AGG GGG CAA TCC GCC GCA GTG T T G - A P F G G L K S M T G F GCT CCA TTC GGC GGC CTC AAA TCC ATG ACT GGA TTC G - P V K K V N T D I T S I CCA GTG AAG AAG GTC AAC ACT GAC ATT ACT TCC ATT - T S N G G R V K C ACA AGC AAT GGT GGA AGA GTA AAC TGC
M A A S S S S S M A L S ATG GCC GCA TCA TCA TCA TCA TCC ATG GCT CTC TCT - S P T L A G K Q L K L N TCT CCA ACC TTG GCT GGC AAG CAA CTC AAG CTG AAC - P S S Q E I G A A R P T CCA TCA AGC CAA GAA TTG GGA GCT GCA AGG TTC ACC
______________________________________ Internal Code/ Plasmid Taxonomic Designation in strain DSM ______________________________________ No. AZ 1/ p PSR 6 delta R V 3161 E. coli K12 and VII AZ 2/ p I-22 3162 E. coli K12 and VII AZ 3/ p II-4 3163 E. coli and VII AZ 4/ pGV 710 3164 E. coli K12 and VII AZ 5/ pGV 3850::pSNIPP 3165 AGR. TUMEF. VII AZ 6/ pGV 3850::pSNIF 3166 AGR. TUMEF. AZ 7/ pGV 3850 3167 AGR. TUMEF. VII ______________________________________