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United States Patent | 6,046,173 |
April 4, 2000 |
The present invention relates to biologically useful material in the form of a pseudocapsid formed from papovavirus major capsid antigen and excluding minor capsid antigens, which pseudocapsid incorporates exogenous material. The invention also relates to a method of using the pseudocapsids as vectors for exogenous material transfer, especially gene transfer in the therapy of genetic disorders, or other uses such as antibody or drug targeting.
Inventors: | Forstova ; Jitka (Prague, CZ); Griffin; Beverly Elayne (London, GB); Krauzewicz; Nina Sheila (Reading, GB) |
Assignee: | Caduceus Limited (London, GB) |
Appl. No.: | 861827 |
Filed: | May 23, 1997 |
Current U.S. Class: | 514/44 |
Intern'l Class: | A61K 048/00 |
Field of Search: | 514/44 |
5437951 | Aug., 1995 | Lowy et al. | 435/69. |
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______________________________________ DISEASE CAUSED BY SINGLE GENE DEFECTS: CURRENT TARGETS FOR GENE THERAPY Disease Defective gene ______________________________________ Immunodeficiency Adenosine deaminase Purine nucleoside phophorylase Hypercholesterolaemia LDL receptor Haemophilia Factor IX Factor VIII Gaucher's disease Glucocerebrosidase Mucopolysaccharidosis .beta.-glucuronidase Emphysema .alpha.-antitrypsin Cystic fibrosis Cystic fibrosis transmembrane regulator Phenylketonuria Phenylalanine hydroxylase Hyperammonaemia Orinthine transcarbamylase Citrullinaemia Arginosuccinate synthetase Muscular dystrophy Dystrophin Thalassaemia .beta.-globin Sickle cell anaemia .beta.-globin Leukocyte adhesion deficiency CD-18 ______________________________________
TABLE 1 ______________________________________ Comparison of Efficiency of Formation of Dense Foci. Method of DNA Efficiency of transfer transfer No. of foci per dish (foci/microgram DNA) ______________________________________ Absorption of 0 0 "naked" DNA Calcium phosphate 15 7.5 transfection Pseusocapsid 57 570* transfer ______________________________________ *Calculation based on packaging efficiency 5%.