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United States Patent | 6,008,005 |
Shiraki ,   et al. | December 28, 1999 |
A method for estimation of change in bone mineral density or a method for diagnosis of osteoporosis, comprising the step of measuring change in a concentration of soluble interleukin-6 receptor in a blood sample, by for example, sandwich assay or competition assay; and a kit for carrying out said methods, comprising: (1) an anti-sIL-6R antibody immobilized to a solid carrier, and (2) an anti-sIL-6R antibody is bound to a detectable marker or capable of binding to a detectable marker.
Inventors: | Shiraki; Masataka (Minamiazumi-gun, JP); Chen; Jui-Tung (Tokyo, JP); Morita; Ikuo (Tokyo, JP); Maruo; Naoko (Yokohama, JP) |
Assignee: | Tosoh Corporation (Shinnanyo, JP) |
Appl. No.: | 603121 |
Filed: | February 20, 1996 |
Feb 23, 1995[JP] | 7-035349 |
Intern'l Class: | G01N 033/53; G01N 021/76; C07K 016/00 |
Field of Search: | 435/7.2,7.92,7.94,69.1,69.52,328,334,335 530/350,300,395,388.22,389.2,391.1,391.3 436/518,524,527,63,172 |
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5171840 | Dec., 1992 | Kishimoto | 530/350. |
5223611 | Jun., 1993 | Kishimoto | 530/351. |
5480796 | Jan., 1996 | KIshimoto | 435/240. |
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TABLE 1 ______________________________________ Correlation coefficient ______________________________________ Serum sIL-6R 0.400 Serum osteocalcin 0.327 Urine deoxypyridinoline 0.375 (creatinine-corrected) ______________________________________