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United States Patent | 5,776,773 |
Bruggemann | July 7, 1998 |
Embryonic stem cells that are essentially free of yeast DNA are prepared from suitably marked yeast artificial chromosomes and used to transfer DNA segments of considerable size into organisms.
Inventors: | Bruggemann; Marianne (Foxton, GB) |
Assignee: | The Babraham Institute (Cambridge, GB) |
Appl. No.: | 204294 |
Filed: | March 7, 1994 |
PCT Filed: | September 10, 1992 |
PCT NO: | PCT/GB92/01651 |
371 Date: | March 7, 1994 |
102(e) Date: | March 7, 1994 |
PCT PUB.NO.: | WO93/05165 |
PCT PUB. Date: | March 18, 1993 |
Sep 10, 1991[GB] | 9119338 |
Current U.S. Class: | 435/325; 435/320.1; 435/449 |
Intern'l Class: | C12N 005/16; C12N 015/06; C12N 015/64 |
Field of Search: | 435/240.2,240.26,172.3,172.2,320.1,325 |
Foreign Patent Documents | |||
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8801648 | Mar., 1988 | WO. | |
8810118 | Oct., 1988 | WO. | |
8912823 | Dec., 1989 | WO. | |
9004036 | Apr., 1990 | WO. | |
9203918 | Mar., 1992 | WO. |
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