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United States Patent | 5,763,195 |
Wood ,   et al. | June 9, 1998 |
The complete amino acid and nucleotide sequence for adenylate cyclase stimulating factor is provided, thereby facilitating the synthesis of ACSF in recombinant cell culture. ACSF amino acid sequence variants and ACSF antibodies are provided which are useful in the treatment of humoral hypercalcemia of malignancy or in immunoassays for ACSF. In particular, antibodies capable of binding only the C-terminal domains of ACSF are useful in immunoassays for ACSF which avoid interference by parathyroid hormone. Also provided are novel polypeptides selected from the group of the ACSF basic peptide, the ACSF C-terminal peptide, or the ACSF domain containing both of the basic and C-terminal peptides.
Inventors: | Wood; William I. (San Mateo, CA); Martin; Thomas John (Kew, AU); Suva; Larry John (East Kew, AU) |
Assignee: | Genentech, Inc. (South San Francisco, CA); University of Melbourne (Victoria, AU) |
Appl. No.: | 425491 |
Filed: | April 20, 1995 |
Current U.S. Class: | 435/7.4; 424/130.1; 424/138.1; 424/158.1; 435/7.1; 435/7.23; 435/7.71; 435/7.9; 435/7.92; 435/7.93; 435/7.94; 435/7.95; 530/387.1; 530/387.7; 530/388.23 |
Intern'l Class: | G01N 033/53; G01N 033/573; C07K 016/30; A61K 039/395 |
Field of Search: | 530/387.1,387.7,388.23 435/7.94,7.1,7.4,723,7.92,7.93,7.71,7.9 424/130.1,138.1,158.1 |
4703008 | Oct., 1987 | Lin | 435/240. |
4968669 | Nov., 1990 | Rosenblatt et al. | 435/240. |
5114843 | May., 1992 | Rosenblatt et al. | 435/7. |
5116952 | May., 1992 | Martin et al. | 530/399. |
5149779 | Sep., 1992 | Chorev et al. | 530/317. |
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TABLE 1 ______________________________________ Original Residue Exemplary Substitutions ______________________________________ Ala gly; ser Arg lys Asn Gln; his Asp glu Cys ser Gln asn Glu asp Gly ala His asn gln Ile leu; val Leu ile; val Lys arg; gln; glu Met leu; ile Phe met; leu; tyr Ser thr Thr ser Trp tyr Tyr trp; phe Val ile; leu ______________________________________