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United States Patent | 5,747,249 |
Smith ,   et al. | May 5, 1998 |
A process for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations wherein chromophores or fluorophores are used to tag the DNA fragments produced by the sequencing chemistry and permit the detection and characterization of the fragments as they are resolved by electrophoresis through a gel. Preferably four different fragment sets are tagged with the fluorophores fluorescein, Texas Red, tetramethyl rhodamine, and 7-nitro-benzofurazan. A system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations comprising: a source of chromophore or fluorescent tagged DNA fragments; a zone for contacting an electrophoresis gel; means for introducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.
Inventors: | Smith; Lloyd M. (Madison, WI); Hood; Leroy E. (Seattle, WA); Hunkapiller; Michael W. (San Carlos, CA); Hunkapiller; Tim J. (Seattle, WA); Connell; Charles R. (Redwood City, CA) |
Assignee: | California Institute of Technology (Pasadena, CA) |
Appl. No.: | 361176 |
Filed: | December 21, 1994 |
Intern'l Class: | C12Q 001/68; C12P 019/34; C07H 021/04 |
Field of Search: | 435/6,91.1 536/25.3,25.31,25.32,24.3 |
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TABLE 1 ______________________________________ Reverse Phase HPLC Conditions for Dye-oligonucleotide Purification Sample Retention time ______________________________________ PLP-15.sup.a 18' PLP-15-T-NH.sub.2 .sup.b 18' FITC PLP-15.sup.c 27' NBD PLP-15 25' TMRITC PLP-15 32' and 34'.sup.d Texas Red PLP-15 42' ______________________________________