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United States Patent | 5,641,641 |
Wood | June 24, 1997 |
Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions. Thus, the invention provides methods, compositions and test kits for assaying samples for the presence of a beetle luciferase or, using a beetle luciferase-luciferin reaction, the presence of ATP. The invention also provides the complex of the coenzyme, Coenzyme A, a beetle luciferase and oxyluciferin in its exited state in the luciferase-luciferin reaction and luciferyl-CoA, the thioester of Coenzyme A and luciferin (D-(-)-2-(6'-hydroxy -2'-benzothiazolyl)-.DELTA..sup.2 -thiazoline-4-carboxylic acid).
Inventors: | Wood; Keith V. (Madison, WI) |
Assignee: | Promega Corporation (Madison, WI) |
Appl. No.: | 485834 |
Filed: | June 7, 1995 |
Current U.S. Class: | 435/8; 435/4; 435/184; 435/810; 435/968; 435/975; 436/164; 436/166; 436/172; 436/808 |
Intern'l Class: | C12Q 001/66; G01N 033/53 |
Field of Search: | 435/8,4,184,810,968,975 436/164,166,172,808 |
4234681 | Nov., 1980 | Deluca-McElroy | 435/8. |
4235961 | Nov., 1980 | Lundin et al. | 435/8. |
4246340 | Jan., 1981 | Lundin et al. | 435/8. |
4614712 | Sep., 1986 | Baldwin et al. | 435/4. |
4665022 | May., 1987 | Schoeffer et al. | 435/8. |
4833075 | May., 1989 | Vijayalakshmi et al. | 435/8. |
Foreign Patent Documents | |||
0 299 601 | Jan., 1989 | EP. | |
0 309 184 | Mar., 1989 | EP. | |
2026156 | Jan., 1980 | GB. |
Webster et al, Biolumin. Chemilumin, 2nd, pp. 499-504, 1981. Shimomura, Symp. Soc. Exp. Biol., vol. 39, pp. 351-372, 1985. Airth et al., "The Function of Coenzyme A in Luminescence," Biochim. et Biophys. Acta 27, 519-532 (1958). Rhodes and McElroy, "The Synthesis and Function of Luciferyl-Adenylate and Oxyluciferyl-Adenylate," J. Biol. Chem. 233, 1528-1537 (1958). McElroy and Seliger, "The Chemistry of Light Emission," Advances Enzymol. 25, 119-166 (1963). Hammerstedt, "An Automated method for ATP analysis utilizing the luciferin-luciferase reaction," Anal. Biochem. 52, pp. 449-455 (1973). Webster, et al., "Optimization of the Firefly Luciferase Assay for ATP," J. Appl. Biochem. 2, pp. 469-479 (1980). Sillero, et al., "Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase", Eur. J. Biochem, vol. 202, pp. 507-513 (1991). Gunther et al, Eur. J. Biochem, vol. 202, No. 2, pp. 507-513, 1991. Wood, Chemical Abstracts, vol. 117, p. 365, Ref. #1273631C, 1992 (Chemilumin. Proc. Int. Symp. 6th. 1990, 11-14). |