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United States Patent | 5,580,716 |
Johnston ,   et al. | December 3, 1996 |
A method for conferring resistance to a parasite to a host of the parasite, which comprises isolating a gene fragment from the parasite and inserting the gene fragment or a DNA or RNA segment substantially homologous to the gene fragment or to a DNA or RNA sequence functionally equivalent to the gene fragment into the host, wherein (1) transcription of the gene fragment or the DNA or RNA segment in the host occurs in an anti-sense direction, (2) the gene fragment or the DNA or RNA segment is expressed as a gene product in the host, wherein the gene product is capable of disrupting an essential activity of the parasite, or (3) the gene fragment or the DNA or RNA segment is a binding site capable of competing with a native binding site in the parasite, is disclosed along with hosts produced by this process. Particularly preferred is conferring resistance using a gene fragment from a replicase gene of an RNA virus.
Inventors: | Johnston; Stephen A. (309 N. Gregson, Durham, NC 27701); Sanford; John C. (Geneva, NY) |
Assignee: | Johnston; Stephen A. (Dallas, TX); Cornell Research Foundation, Inc. (Ithaca, NY) |
Appl. No.: | 344616 |
Filed: | November 17, 1994 |
Current U.S. Class: | 435/5; 435/6; 435/69.1; 435/252.3; 435/320.1; 800/280 |
Intern'l Class: | C12Q 001/70; C12Q 001/68; C12N 005/14; C12N 015/66 |
Field of Search: | 514/44 435/172.3,320.1,240.1,240.2,240.4,252.33,69.1,5,6 |
Foreign Patent Documents | |||
0110385 | Jun., 1984 | EP. | |
0140308 | May., 1985 | EP. | |
0194809 | Sep., 1986 | EP. | |
2148302A | May., 1985 | GB. | |
83/01451 | Apr., 1983 | WO. | |
8504898 | Nov., 1985 | WO. |
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TABLE 1 ______________________________________ Strain + Plasmid Inducer # of Plaques Size ______________________________________ GM1 -- + 300 normal puR222 - 360 " " + 348 " Hpa (antisense) - 247 " " + 263 small Hpa (sense) - 224 normal " + 234 " pGL101 - 176 very small " + 101 very very small ______________________________________
TABLE I ______________________________________ No. of plaques/plate Strain with Plasmid -IPTG +IPTG f2 ______________________________________ PUC9 240 2400 172 2290 294 600 116 #7 250 87 332 1000 bp #10 166 53 348 #11 264 16 279 #4 243 36 277 500 bp #5 208 144 266 #8 240 1342 215 ______________________________________