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United States Patent | 5,567,607 |
Zhao ,   et al. | October 22, 1996 |
A method is provided for making transgenic animals by transferring DNA into embryo by subjecting the mixture of the DNA to be transferred and the embryo to an electric discharge.
Inventors: | Zhao; Xi (Saratoga, CA); Wong; Tai-kin (Saratoga, CA) |
Assignee: | Incell (Santa Clara, CA) |
Appl. No.: | 424221 |
Filed: | April 19, 1995 |
Current U.S. Class: | 800/21; 435/173.6; 435/320.1 |
Intern'l Class: | C12N 015/85; C12N 005/16; C12N 013/00 |
Field of Search: | 800/2,DIG. 1,DIG. 2,DIG. 3,DIG. 4 435/172.3,172.1,320.1,240.2,289,173.5,173.6 935/23,32,52,53,54,50 |
4663292 | May., 1987 | Wong et al. | 435/285. |
4870009 | Sep., 1989 | Evans et al. | 435/69. |
Robl et al. 1986 Thenogenobgy 25, 189. Berg et al. 1984 Bioelectrochem. Bioenerget. 12, 119-133. Uize et al. 1987 Gene 55, 339-344. Old et al. 1985 in: Principles of Gene Manipulation An Introduction to Genetic Engineering. Blackwell Sci. Publ., Oxford Eng. pp. 262-265. Hogan et al. 686 in: Manipulating the Main Embryo. A Laboratory Manual. Cold Spring Harbor Laboratory, NY. pp. 153-154. Nemec et al. 1989 Theriogenology 31, 233. Webster's Third New International Dictionary, 1963, G & C Marriam Company Ma. p. 740. Webster's II New Riverside University Dictionary, Soukhanov et al. (ed.) 1984, Houghton Mifflin Company, Boston, MA, pp. 67 and 119-120. Asubel et al. (eds.) 1988, in: Current Protocols in Molecular Biology (vol. 2), John Wiley & Sons, New York, NY, appendix p. A.2.3. |