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United States Patent | 5,552,309 |
March | September 3, 1996 |
A process for introducing an expression vehicle (e.g., plasmids, retroviral vectors, adenoviral vectors) into cells, which comprises contacting the cells with the expression vehicle and a polyol. The polyol may be a polyoxalkylene block copolymer, such as a polyoxypropylene-polyoxyethylene block copolymer. The use of the polyol provides for greater efficiency of transduction of the expression vehicle.
Inventors: | March; Keith L. (Carmel, IN) |
Assignee: | Indiana University Foundation (Bloomington, IN) |
Appl. No.: | 315974 |
Filed: | September 30, 1994 |
Current U.S. Class: | 435/456; 424/93.1; 424/93.2; 424/426; 435/235.1; 435/320.1; 435/458; 514/44 |
Intern'l Class: | A01N 063/00; C12N 005/00; C12N 015/00 |
Field of Search: | 424/93.1,93.2,426 435/172.3,320.1,240.2 514/44 935/57 |
5118512 | Jun., 1992 | O'Leary et al. | 424/549. |
5298222 | Mar., 1994 | O'Leary et al. | 422/28. |
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