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United States Patent | 5,310,463 |
Dadoo ,   et al. | May 10, 1994 |
An electrophoretic or chromatographic separation capillary containing a fluid defines a bore therein through which a sample travels and separates into components. The tube has a side wall defining a through hole therein which is surrounded by a medium including a substance. The substance is introduced into the capillary through the hole by means of gravity, pressure or electroosmosis. The substance introduced may be used to label sample components to enhance detection, or to enhance separation of sample components.
Inventors: | Dadoo; Rajeev (Stanford, CA); Colon; Luis A. (Menlo Park, CA); Fishman; Harvey A. (Stanford, CA); Shear; Jason B. (Menlo Park, CA); Zare; Richard N. (Stanford, CA) |
Assignee: | Board of Trustees of the Leland Stanford Junior University (Stanford, CA) |
Appl. No.: | 975850 |
Filed: | November 13, 1992 |
Current U.S. Class: | 204/451; 73/61.53; 73/61.58; 204/455; 204/601; 204/605; 210/198.2; 210/659 |
Intern'l Class: | G01N 027/26; G01N 027/447 |
Field of Search: | 204/299 R,180.1 210/198.2,656 73/61.58,61.56,61.55,61.53,61.52 |
4394263 | Jul., 1983 | Dosch et al. | 210/198. |
4837161 | Jun., 1989 | Stevens et al. | 210/198. |
5073239 | Dec., 1991 | Hjerten | 204/180. |
5110431 | May., 1992 | Moring | 204/180. |
Foreign Patent Documents | |||
03-144361 | Jun., 1991 | JP | 204/198. |
Stephen L. Pentoney, Jr. et al "On-line Connector for Microcolumns: Application to the On-Column o-Phthaldialdehyde Derivatization of Amino Acids Separated by Capillary Zone Electrophoresis" Analytical Chemistry vol. 60, No. 23(1988) 2625-2629. "Coupled Fused Silica Capillaries for Rapid Capillary Zone Electrophoresis of Proteins," by Nashabeh et al., Journ. of High Res. Chromatogr., 15:289-292 (May 1992). "Post-Capillary Fluorescence Detection in Capillary Zone Electrophoresis," by Rose, Jr. et al., Journ. of Chromatogr., 447:117-131 (1988). "Fluorescence Detection in Capillary Electrophoresis: Evaluation of Derivatizing Reagents and Techniques," by Albin et al., American. Chem. Soc., 63(5):417-422 (Mar. 1, 1991). "On-Column Connector for Microcolumns: Application to the o-Phthaldialdehyde Derivatization of Amino Acids . . . ," by Pentoney, Jr., et al., American Chem. Soc., 60(23):2625-2629 (Dec. 1, 1988). "Direct Control of the Electroosmosis in Capillary Zone Electrophoresis . . . ," by Lee, et al., American Chem. Soc., 62:1550-1552 (Jul. 15, 1990). "Factors Affecting Direct Control of Electroosmosis Using an External . . . ," by Lee et al., American Chem. Soc., 63:1519-1523 (Aug. 1, 1991). "Electroosmotic Flow Control and Monitoring with an Applied Radial . . . ," by Hayes et al., American Chem. Soc., 64:512-516 (Mar. 1, 1992). "On-Column Sample Concentration Using Field Amplification in CZE," by Chien et al., Anal. Chem., 64(8):489-496 (Apr. 15, 1992). Article entitled "Capillary-Zone Electrophoresis with Fraction Collection . . . ," by Takigiku et al., CZE for Desorption MS. |