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United States Patent | 5,302,515 |
Goodwin, Jr. | April 12, 1994 |
The present invention is a single site or multiple site chemotaxis test apparatus or chemotaxis chamber for measuring the effect of concentration gradients of diffusible chemicals upon the directional response of biological cells. The chemotactic test apparatus of the present invention comprise two compartments separated by a thin passage. The dimensions of the thin passage are selected such that cells cannot flow through the thin passage, but must deform themselves by flattening out and then "crawl" or migrate through the passage. These chambers can be used to study the migration or orientation of mobile adherent or non-adherent cells.
Inventors: | Goodwin, Jr.; Richard H. (Bethesda, MD) |
Assignee: | Neuro Probe, Inc. (Cabin John, MD) |
Appl. No.: | 932976 |
Filed: | August 20, 1992 |
Current U.S. Class: | 435/29; 422/101; 422/947; 435/288.5; 435/305.2 |
Intern'l Class: | C12Q 001/02; C12M 003/04; C12M 001/18; C12M 001/20; B01L 011/00 |
Field of Search: | 435/29,285,300,301 422/101 |
3888770 | Jun., 1975 | Avital et al. | 210/238. |
3929583 | Dec., 1975 | Sharpe et al. | 435/301. |
4317726 | Mar., 1982 | Shepel | 210/236. |
4493815 | Jan., 1985 | Fernwood et al. | 422/101. |
4514495 | Apr., 1985 | Schalkowsky et al. | 435/32. |
4714674 | Dec., 1987 | Palladino | 435/18. |
4912057 | Mar., 1990 | Guirguis et al. | 435/285. |
5023173 | Jun., 1991 | Horwitz et al. | 435/29. |
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