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United States Patent | 5,254,464 |
Takeuchi ,   et al. | October 19, 1993 |
It is possible to produce and accumulate D-alanine selectively and to improve the amount of production and accumulation of D-alanine by cultivating a microorganism having both an ability to produce D-alanine and a resistance to D-cycloserine and belonging to the genus Brevibacterium.
Inventors: | Takeuchi; Masae (Aichi, JP); Yonehara; Tetsu (Aichi, JP) |
Assignee: | Toray Industries, Inc. (JP) |
Appl. No.: | 742147 |
Filed: | August 1, 1991 |
Oct 07, 1987[JP] | 62-253127 |
Current U.S. Class: | 435/116; 435/252.1; 435/252.8; 435/840; 435/849 |
Intern'l Class: | C12P 013/06; C12N 015/00; C12N 001/20 |
Field of Search: | 435/252.8,252.1,116,840,849,172.1 |
3117915 | Jan., 1964 | Shiio | 435/843. |
3871959 | Mar., 1975 | Chibata | 435/116. |
Foreign Patent Documents | |||
42-20683 | Oct., 1967 | JP. |
Adelberg et al, Biochemical and Biophysical Research Communications, vol. 18, No. 5-6, 1965. Chemical Abstracts, vol. 67 No. 15, Oct. 9, 1967, p. 6700, abstract No. 71293s. Patent Abstracts of Japan, vol. 8, No. 232(C-248)(1669), Oct. 25, 1984. Chemical Abstracts, vol. 87, No. 7 Aug. 15, 1977, p. 200, Abstract No. 49947z. Biotechnology of Amino Acid Production, Progress in Industrial Microbiology, vol. 24, 1986, pp. 3-13. J. Gen. Appl. Microbiol., vol. 17, No. 2, 1971, pp. 169-172. David, H. L. Appl. Microbiol. vol. 21, 1971, pp. 888-892. Robbins et al., J. Bacteriol. vol. 116, 1973, pp. 12-18. M. Goodfellow et al ed., The Biology of the Actinomycetes, 1984 Academic Press, London pp. 30-31, 44-46 and 77-79. Brock et al., Biology of Microorganisms 1984 pp. 304-310. Clark et al, "D-Cycloserine-Induced Alterations in the Transport of D-Alanine and Glycine in Bacillus subtilis 168" Antimicrob. Agents and Chemo. May 1977, pp. 877-880. Clark et al, "Inducible Resistance to D-cycloserine in Bacillus subtilis 168", Antimicrob. Agents and Chemo., May 1977, pp. 871-876. |
TABLE 1 ______________________________________ Relative growth degree (%) Concentration of D-cycloserine added (mg/l) Strain 0 10 50 ______________________________________ Parent strain 100 36.7 0 Brevibacterium (60.0) (22.0) (0) lactofermentum ATCC 13869 Strain of the 100 78.4 61.8 present invention (87.0) (68.2) (53.8) Brevibacterium lactofermentum DSCR-26 Strain of the 100 -- 109 present invention (92.9) (101.7) Brevibacterium lactofermentum DCSR 17-2 Parent strain 100 87 82 Brevibacterium (125.2) (109.0) (103.0) flavum ATCC 13826 Strain of the 100 103 107 present invention (103.1) (106.6) (110.8) Brevibacterium flavum DCSR 2-2 ______________________________________ Notice: Optical densities at 660 nm of culture broths were shown in (). The relative growth degree as calculated by using the optical density of the culture broth where no Dcycloserine was added as 100%.
TABLE 2 ______________________________________ Amount of D-alanine Strain accumulated Purity* ______________________________________ Comparative Brevibacterium 9.2 g/l 76% example lactofermentum ATCC 13869 Example Brevibacterium 13.2 g/l 100% of the lactofermentum present DCSR-26 invention ______________________________________ ##STR1##
TABLE 3 ______________________________________ Amount of D-alanine D/L Strain accumulated Purity ratio* ______________________________________ Comparative Brevibacterium 1.7 g/l 85% 6.96 example flavum ATCC 13826 Example Brevibacterium 1.9 g/l 95% 13.4 of the flavum present DCSR 2-2 invention ______________________________________ ##STR2##