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United States Patent | 5,248,619 |
Skold ,   et al. | September 28, 1993 |
A method is disclosed for conducting an assay for an analyte. The method comprises causing a specific binding pair member in a first aqueous medium to become bound to a first bibulous member by contacting a portion of the first bibulous member with the medium. The first bibulous member is in liquid receiving relationship with an absorbent member. The contacting is carried out under conditions wherein the first medium traverses the first bibulous member and at least a portion of the absorbent member by capillary action. The method further comprises causing the first bibulous member to come into liquid receiving relationship with a second bibulous member. A reagent in a second aqueous medium is absorbed by and preferably becomes non-diffusively bound to the second bibulous member in relation to the presence of analyte in the first medium. This is accomplished by contacting a portion of the first bibulous member with the second aqueous medium under conditions wherein the second aqueous medium traverses the first bibulous member by capillary action and at least a portion of the second bibulous member. Next, the presence of the reagent on at least a portion of the second bibulous member is determined.
Inventors: | Skold; Carl N. (Mountain View, CA); Shanafelt; Armen B. (San Francisco, CA); Ghazarossian; Vartan (Menlo Park, CA); Ullman; Edwin F. (Atherton, CA) |
Assignee: | Syntex (U.S.A) Inc. (Palo Alto, CA) |
Appl. No.: | 714791 |
Filed: | June 13, 1991 |
Current U.S. Class: | 436/514; 422/56; 422/61; 435/7.92; 435/970; 435/975; 436/518 |
Intern'l Class: | G01N 033/543; G01N 033/558 |
Field of Search: | 422/56,61 435/7.92,805,810,970,975 436/514,518,808,810 |
4108729 | Aug., 1978 | Mennen | 422/56. |
4826759 | May., 1989 | Guire et al. | 435/4. |
4883764 | Nov., 1989 | Kloepfer | 436/63. |
4999287 | Mar., 1991 | Allen et al. | 435/11. |
TABLE 1 ______________________________________ Digoxin Concentration Migration Height (ng/mL) (mm) ______________________________________ 0 20 0.5 24 1 29 2 39 3 48 4 55 ______________________________________