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United States Patent | 5,234,911 |
Christophers ,   et al. | August 10, 1993 |
A substance is prepared having interleukin-8 inhibiting activity by extracting psoriatic scales and separating the extract on several successive HPLC columns, using an assay for IL-8i activity based on the inhibition of myeloperoxidase release and/or of the neutrophil chemotaxis of a reference sample. The substance is suitable for the preparation of anti-inflammatory medicines.
Inventors: | Christophers; Enno (Kiel, DE); Schroder; Jens-Michael (Blumenthal, DE) |
Assignee: | Gist-brocades, N.V. (Delft, NL) |
Appl. No.: | 642119 |
Filed: | January 16, 1991 |
Jul 09, 1990[EP] | 90201841.5 |
Current U.S. Class: | 514/21; 514/12; 530/350; 530/351; 530/412; 530/842 |
Intern'l Class: | A61K 037/02; C07K 003/02; C07K 015/06 |
Field of Search: | 530/414,412,350,351,842 514/12,21 424/85.2 |
5079228 | Jan., 1992 | Cohen et al. | 514/3. |
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______________________________________ PMN polymorphonuclear leukocytes, including neutrophils PBS phosphate buffered saline BSA bovine serum albumine TFA trifluoroacetic acid MPO myeloperoxidase ______________________________________ Lit. ref. (1): J. Immunol. 1987, 139, p. 3474.