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United States Patent | 5,210,019 |
Margalit | May 11, 1993 |
A screening assay for detecting the presence of Pseudomonas bacteria in a test sample comprising isolating the bacteria present in the sample, extracting Azurin protein from the periplasm within the bacteria, reacting the extract with an enzymatically labelled, Azurin-specific antibody and a color-producing substrate specific for the enzyme which is capable of producing a color distinct from the color generated by Azurin and reading the results of the reaction of the sample with the antibody to determine whether the color has been produced whereby the presence of Pseudomonas bacteria in the sample is indicated.
Inventors: | Margalit; Ruth (San Marino, CA) |
Assignee: | The United States of America as represented by the Administrator of the (Washington, DC) |
Appl. No.: | 501908 |
Filed: | March 30, 1990 |
Current U.S. Class: | 435/7.32; 435/7.92; 435/874; 436/518 |
Intern'l Class: | G01N 033/569 |
Field of Search: | 435/7.32,7.92,7.94,961 436/530,518 |
4963480 | Oct., 1990 | Belcour et al. | 435/42. |
Stahey et al., J. Bacteriol., vol. 43, No. 2, pp. 149-154, (1942). Trees et al., J. Inf. Dis., vol. 161, pp. 336-339. (1990). A. B. Champion, K. L. Soderbere, & A. C. Wilson, "Immunological Comparison of Azurins of Known Amino Acid Sequence," J. Mol. Evol. 5, 291-305 (1975). Nenad M. Kostic, Ruth Margalit, Chi-Ming Che & Harry B. Gray, "Kinetics of Long-Distance Ruthenium-to-Copper Electron Transfer in (Pentaammineruthenium histidine-83) Azurin", Journal of the American Chemical Society, 1983, 105, 7765. D. Y. Mason, M. Naiem, Z. Abdulaziz, J. R. G. Nash, K. C. Gatter & H. Stein, Immuno histological Applications of Monoclonal Antibodies, pp. 585-613. Joseph S. Lam, Lucy M. Mutharia & Robert E. W. Hancock, "Application of Monoclonal Antibodies to the Study of te Surface of Antigens in Pseudomonas aeruginosa, Monoclonal Antibodies Against Bacteria," vol. II, 143-157. |