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United States Patent | 5,091,652 |
Mathies ,   et al. | February 25, 1992 |
A fluorescent scanner for scanning the fluorescence from a fluorescence labeled separated sample on a sample carrier including a confocal microscope for illuminating a predetermined volume of the sample carrier and/or receiving and processing fluorescence emissions from said volume to provide a display of the separated sample.
Inventors: | Mathies; Richard A. (Contra Costa, CA); Peck; Konan (Contra Costa, CA) |
Assignee: | The Regents of the University of California (Oakland, CA) |
Appl. No.: | 531900 |
Filed: | June 1, 1990 |
Current U.S. Class: | 250/458.1; 204/612; 250/459.1; 250/461.1; 250/461.2 |
Intern'l Class: | G01N 021/64 |
Field of Search: | 250/234,461.2,461.1,459.1,458.1 |
4087685 | May., 1978 | Froot | 250/302. |
4284897 | Aug., 1981 | Sawamura et al. | 250/461. |
4354114 | Oct., 1982 | Karnaukhov et al. | 250/458. |
4734578 | Mar., 1988 | Horikawa | 250/234. |
4791310 | Dec., 1988 | Honig et al. | 250/458. |
4893008 | Jan., 1990 | Horikawa | 250/234. |
5022757 | Jun., 1991 | Modell | 356/318. |
Foreign Patent Documents | |||
WO86/05587 | Sep., 1986 | WO | 250/461. |
Mathies, R. A. and Stryer, L. (1986), Applications of Fluorescence in the Biomedical Sciences, Eds. Taylor, D. L., Waggoner, A.SA., Lanni, F. Murphy, R. F., and Birge, R. (Alan R. Liss, Inc., New York) pp. 129-140. Nguyen, D. C.; Keller R. A.; Jett, J. H.; Martin, J. C. (1987), Detection of Single Molecules of Phycoerythrin in Hydrodynamically Focused Flows by Laser-Induced Fluorescence, Anal. Chem. 59, 2158-2161. Peck, K.; Stryer, L.; Glazer, A. N.; Mathies, R. A. (1989) Single molecule fluorescence detection: Autocorrelation criterion and experimental realization with phycoerythrin Proc. Natl. Acad. Sci. U.S.A., 86, 4087-4091. Mathies, R. A.; Peck, K.; Stryer, L. (1990), Optimization of High-sensitivity Fluorescence Detection, submitted for publication. Glaser, A. N.; Peck, K.; Mathies, R. A. (1990) A stable double-stranded DNA--ethidium homodimer complex: Application to picogram fluorescence detection of DNA in agarose gels, submitted for publication, Proc. Nat. Acad. Sci. U.S.A. Ansorge, W.; Rosenthal, A.; Sproat, B.; Schwager, C.; Stegemann, J.; & Voss, H. (1988) Non-radioactive automated sequencing of oligonucleotides by chemical degradation, Nucleic Acids Research vol. 16, 2203-2207. Smith, L. M.; Sanders, J. Z.; Kaiser, R. J.; Hughes, P.; Dodd, C.; Connell, C. R.; Heiner, C.; Kent, S. B. H. & Hood, L. E. (1986), Fluorescence detection in automated DNA sequence analysis, Nature, vol. 321, 674-679. Sanger, F.; Nicklen, S.; & Coulson, A. R. (1977), DNA sequencing with chain-terminating inhibitors, Proc. Natl. Acad. Sci., vol. 74, No. 12, pp. 5463-5467. |